Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,774,321	T→C	V178A (GTC→GCC)	ydiB →	shikimate dehydrogenase/quinate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,774,321	0	T	C	100.0%	72.9 / NA	21	V178A (GTC→GCC)	ydiB	shikimate dehydrogenase/quinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/10);  total (11/10)

TTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAA  >  NC_000913/1774249‑1774393                                                                         |                                                                         ttAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACgg                                                                  >  1:316980/1‑81 (MQ=255)  tAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGt                                                                 >  2:316986/1‑81 (MQ=255)        tCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGa                                                           <  1:316959/81‑1 (MQ=255)            gATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGAtctc                                                       >  1:317010/1‑81 (MQ=255)                   tctacGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGAt                                                 >  2:316995/1‑80 (MQ=255)                    cttcGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATc                                                <  2:316960/80‑1 (MQ=255)                           aaaGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGcc                                        >  2:316993/1‑81 (MQ=255)                            aaGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCt                                       >  2:316979/1‑81 (MQ=255)                            aaGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCt                                       >  1:316990/1‑81 (MQ=255)                                  tCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCt                                  >  2:316997/1‑80 (MQ=255)                                   cGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTg                                 >  1:316996/1‑80 (MQ=255)                                    gCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGa                                <  1:316961/80‑1 (MQ=255)                                      cTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGc                             >  2:316994/1‑81 (MQ=255)                                            gcAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGc                       >  1:316989/1‑81 (MQ=255)                                                   gTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTccgcc                <  1:316962/81‑1 (MQ=255)                                                       aTGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGAc             <  1:316963/80‑1 (MQ=255)                                                       aTGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACa            <  2:316964/81‑1 (MQ=255)                                                        tGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAt           <  2:316965/81‑1 (MQ=255)                                                          aaaaCACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAtt          <  2:316966/80‑1 (MQ=255)                                                           aaaCACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAtttt        <  1:316967/81‑1 (MQ=255)                                                                 cGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCaa  <  1:316968/81‑1 (MQ=255)                                                                         |                                                                         TTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAA  >  NC_000913/1774249‑1774393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑