| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,780,371 | C→T | A252V (GCC→GTC) | ydiQ → | putative electron transfer flavoprotein subunit YdiQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,780,371 | 0 | C | T | 97.3% | 128.4 / ‑5.8 | 37 | A252V (GCC→GTC) | ydiQ | putative electron transfer flavoprotein subunit YdiQ |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (21/15); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACAGAACGTAAGCACATCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGCCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATCCTGAA > NC_000913/1780293‑1780445 | aaaCAGAACGTAAGCACATCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGtcc > 1:317788/1‑81 (MQ=255) aaCAGAACGTAAGCACATCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCct > 1:317791/1‑81 (MQ=255) aaCGTAAGCACATCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCctgaact > 1:317775/1‑81 (MQ=255) aCGTAAGCACATCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCctgaact > 2:317778/1‑80 (MQ=255) cGTAAGCACATCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCctgaactg > 1:317771/1‑80 (MQ=255) acaTCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTAc < 1:317741/81‑1 (MQ=255) atcatTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACgg < 1:317742/81‑1 (MQ=255) aTCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGagaag > 2:317794/1‑80 (MQ=255) aTCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGagaag < 1:317743/80‑1 (MQ=255) aTCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGagaag < 1:317744/80‑1 (MQ=255) tCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGagaaga < 1:317745/80‑1 (MQ=255) aTAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAAc > 2:317776/1‑80 (MQ=255) tAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGa > 2:317783/1‑81 (MQ=255) tAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGa < 1:317746/81‑1 (MQ=255) aaCGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGAt > 1:317787/1‑81 (MQ=255) aCGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGAt < 2:317747/80‑1 (MQ=255) ttCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGt > 1:317777/1‑80 (MQ=255) ttCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGt > 2:317774/1‑80 (MQ=255) gCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAAt > 2:317784/1‑81 (MQ=255) ccATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGc > 2:317780/1‑81 (MQ=255) cATTGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCg > 1:317781/1‑81 (MQ=255) tGCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCt > 2:317789/1‑81 (MQ=255) gCCGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTg > 2:317786/1‑81 (MQ=255) cGAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTggg < 1:317748/81‑1 (MQ=255) gAGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGt > 2:317798/1‑81 (MQ=255) aGCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTc > 1:317792/1‑81 (MQ=255) gCTGGCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCt < 1:317749/81‑1 (MQ=255) ggCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGTGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCtt > 1:317790/1‑79 (MQ=255) gCGGAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCttt < 1:317750/79‑1 (MQ=255) ggAACATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCg < 1:317751/81‑1 (MQ=255) aaCATCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGAt < 1:317752/81‑1 (MQ=255) tCTGAAGAAAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATc > 1:317793/1‑81 (MQ=255) aagaaAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATCCt < 1:317756/79‑1 (MQ=255) aagaaAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATCCt < 1:317755/79‑1 (MQ=255) aagaaAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATCCt < 1:317753/79‑1 (MQ=255) aagaaAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATCCTg > 2:317785/1‑80 (MQ=255) aagaaAGCCCTGAACTGTAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATc < 2:317754/77‑1 (MQ=255) agaaAGTCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATCCTGaa > 1:317800/1‑81 (MQ=255) | AAACAGAACGTAAGCACATCATTATCGATAACGATTCGCCGGAGGCCATTGCCGAGCTGGCGGAACATCTGAAGAAAGCCCTGAACTGAAGCCTACGGAGAAGAAACGATGAGTCAATTAAACAGCGTCTGGGTCTTTAGCGATAATCCTGAA > NC_000913/1780293‑1780445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |