Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,796,866	T→C	Y360C (TAT→TGT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,796,866	0	T	C	100.0%	118.2 / NA	33	Y360C (TAT→TGT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/18);  total (15/18)

GCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCG  >  NC_000913/1796793‑1796940                                                                          |                                                                           gCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTg                                                                     <  1:320182/81‑1 (MQ=255)    gATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTgaga                                                                  >  1:320228/1‑81 (MQ=255)     aTGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGAc                                                                 >  2:320235/1‑81 (MQ=255)          gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                            <  1:320183/81‑1 (MQ=255)          gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                            >  2:320239/1‑81 (MQ=255)          gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                            >  1:320232/1‑81 (MQ=255)            tCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCgg                                                          >  1:320233/1‑81 (MQ=255)            tCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCgg                                                          <  2:320184/81‑1 (MQ=255)              agcagACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTa                                                        >  2:320241/1‑81 (MQ=255)                 agACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGc                                                     <  2:320185/81‑1 (MQ=255)                   aCGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAg                                                   >  1:320246/1‑81 (MQ=255)                    cGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAgg                                                  <  1:320186/81‑1 (MQ=255)                       gTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCa                                               >  1:320236/1‑81 (MQ=255)                          gCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATg                                             <  1:320187/80‑1 (MQ=255)                           cACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                           <  2:320188/81‑1 (MQ=255)                           cACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                           <  1:320189/81‑1 (MQ=255)                            aCGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgacg                                          <  2:320190/81‑1 (MQ=255)                              gTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgacgac                                        <  2:320191/81‑1 (MQ=255)                                 ccATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAg                                     >  2:320250/1‑81 (MQ=255)                                      gCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACCACGGTGAGACGCATCGGTATGCACGCCATGACGACGAGCACGa                                >  1:320248/1‑81 (MQ=255)                                          tgtgCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGAcc                              <  2:320192/79‑1 (MQ=255)                                             gctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGa                         >  2:320249/1‑81 (MQ=255)                                                      ccGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGc                 <  1:320193/80‑1 (MQ=255)                                                        ggATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCggg              >  2:320256/1‑81 (MQ=255)                                                        ggATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCggg              <  2:320194/81‑1 (MQ=255)                                                         gATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGc             <  2:320195/81‑1 (MQ=255)                                                          aTCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCt            <  2:320196/81‑1 (MQ=255)                                                            cAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTa           >  1:320261/1‑80 (MQ=255)                                                             aaCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTaa          <  2:320197/80‑1 (MQ=255)                                                              aCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTa           >  2:320243/1‑78 (MQ=255)                                                                 ccACGCTCACAACGGTGAGACGCTAAGGTATGCAGGCCATGACAACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAc     <  2:320198/81‑1 (MQ=255)                                                                 ccACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAc     <  1:320199/81‑1 (MQ=255)                                                                    cGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAcgcg  >  2:320242/1‑81 (MQ=255)                                                                          |                                                                           GCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCG  >  NC_000913/1796793‑1796940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑