Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,082	T→A	V228V (GTA→GTT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,082	0	T	A	100.0%	110.8 / NA	31	V228V (GTA→GTT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/15);  total (16/15)

GTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAA  >  NC_000913/1830004‑1830158                                                                               |                                                                             gTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAAc                                                                            <  1:324732/81‑1 (MQ=255)    aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCt                                                                          >  1:324773/1‑80 (MQ=255)    aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTg                                                                         <  1:324733/81‑1 (MQ=255)     aaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGa                                                                        >  1:324758/1‑81 (MQ=255)        aTGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGAcc                                                                      >  1:324761/1‑80 (MQ=255)             gATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGAc                                                                >  1:324772/1‑81 (MQ=255)                ataGTTACGGTAGCGAAAACCATCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTc                                                             >  1:324776/1‑81 (MQ=255)                  aGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCt                                                           >  2:324766/1‑81 (MQ=255)                  aGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCt                                                           >  2:324765/1‑81 (MQ=255)                   gTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCt                                                           <  2:324734/80‑1 (MQ=255)                   gTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTg                                                          <  2:324735/81‑1 (MQ=255)                    ttACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTgg                                                         <  1:324736/81‑1 (MQ=255)                     tACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTggg                                                        >  1:324771/1‑81 (MQ=255)                      aCGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGc                                                       >  1:324767/1‑81 (MQ=255)                              gAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTgg                                                >  1:324737/1‑80 (MQ=255)                               aaaaCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGa                                              >  1:324777/1‑81 (MQ=255)                                   ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaa                                          <  1:324738/81‑1 (MQ=255)                                         ttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTgg                                    >  2:324762/1‑81 (MQ=255)                                               aGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAg                              <  2:324739/81‑1 (MQ=255)                                                 cACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAt                            <  2:324740/81‑1 (MQ=255)                                                    cgcgcgGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCgg                         <  2:324741/81‑1 (MQ=255)                                                       gcgGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATg                      >  1:324770/1‑81 (MQ=255)                                                        cgGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGt                     <  2:324742/81‑1 (MQ=255)                                                           gCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTc                  <  1:324743/81‑1 (MQ=255)                                                             aGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCgg                <  1:324744/81‑1 (MQ=255)                                                                cgcgGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCAt             <  1:324745/81‑1 (MQ=255)                                                                 gcgGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCAt             >  1:324769/1‑80 (MQ=255)                                                                    gTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGt         <  2:324746/81‑1 (MQ=255)                                                                         cGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTg     >  1:324783/1‑80 (MQ=255)                                                                         cGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc    <  1:324747/81‑1 (MQ=255)                                                                           gATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCaa  >  2:324778/1‑81 (MQ=255)                                                                               |                                                                             GTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAA  >  NC_000913/1830004‑1830158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑