Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,943	T→C	Y347C (TAC→TGC)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,943	0	T	C	100.0%	115.3 / NA	32	Y347C (TAC→TGC)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

ACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATA  >  NC_000913/1830868‑1831020                                                                            |                                                                              acGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCg                                                                          <  1:324898/81‑1 (MQ=255)      gCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCAtt                                                                     >  1:324938/1‑81 (MQ=255)      gCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCAt                                                                      <  2:324899/80‑1 (MQ=255)       ccACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTc                                                                    <  1:324900/81‑1 (MQ=255)       ccACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTc                                                                    <  1:324901/81‑1 (MQ=255)        cACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGTGATCTGTTTCGCTTGCCCGGCGCAATCGGCAtt                                                                     >  1:324936/1‑79 (MQ=255)               aaTCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTAcaca                                                            <  2:324902/81‑1 (MQ=255)                aTCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAg                                                           <  2:324903/81‑1 (MQ=255)                       ccATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATc                                                     <  1:324904/80‑1 (MQ=255)                            aCGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCaaa                                               >  1:324950/1‑81 (MQ=255)                            aCGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCaaa                                               >  1:324935/1‑81 (MQ=255)                            aCGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCaa                                                <  1:324906/80‑1 (MQ=255)                            aCGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCaa                                                <  1:324905/80‑1 (MQ=255)                               ccTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCt                                            <  1:324907/81‑1 (MQ=255)                                   cTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTaa                                          <  1:324908/79‑1 (MQ=255)                                    tttCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGcc                                       >  2:324931/1‑81 (MQ=255)                                    tttCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGcc                                       >  1:324947/1‑81 (MQ=255)                                      tCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCcgc                                     >  2:324939/1‑81 (MQ=255)                                        gccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCcgcg                                    <  1:324910/80‑1 (MQ=255)                                        gccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCcgcg                                    <  1:324909/80‑1 (MQ=255)                                               ccTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCAc                            >  1:324937/1‑81 (MQ=255)                                                cTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACt                           <  1:324911/81‑1 (MQ=255)                                                  gagagaTCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTg                          >  2:324944/1‑80 (MQ=255)                                                             ttCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAg              >  2:324941/1‑81 (MQ=255)                                                               cGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGc             <  2:324912/80‑1 (MQ=255)                                                                gCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCgg           >  2:324946/1‑81 (MQ=255)                                                                gCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCgg           >  1:324943/1‑81 (MQ=255)                                                                gCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCgg           >  1:324940/1‑81 (MQ=255)                                                                gCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCgg           >  1:324934/1‑81 (MQ=255)                                                                     cccGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGAt       <  1:324913/80‑1 (MQ=255)                                                                        ggCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTaa    >  1:324942/1‑80 (MQ=255)                                                                        ggCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAAt   <  2:324914/81‑1 (MQ=255)                                                                         gcgcAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATa  <  2:324915/81‑1 (MQ=255)                                                                            |                                                                              ACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATA  >  NC_000913/1830868‑1831020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑