Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,832,618	G→C	G64A (GGC→GCC)	xthA →	exodeoxyribonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,832,618	0	G	C	100.0%	63.9 / NA	19	G64A (GGC→GCC)	xthA	exodeoxyribonuclease III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/8);  total (11/8)

TGACGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCG  >  NC_000913/1832541‑1832693                                                                              |                                                                            tGACGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTAtgccgt                                                                          >  1:325163/1‑81 (MQ=255)  gACGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTAtgccgtg                                                                         >  2:325168/1‑81 (MQ=255)     gATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTAtggcgtggcg                                                                      >  1:325166/1‑81 (MQ=255)          gTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCgctgct                                                                 >  2:325172/1‑81 (MQ=255)              ccGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGAc                                                              <  1:325139/80‑1 (MQ=255)                    gAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCaaa                                                          >  1:325161/1‑78 (MQ=255)                     aaGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAgaga                                                      >  1:325167/1‑81 (MQ=255)                      agagGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGAc                                                     >  2:325177/1‑81 (MQ=255)                               gAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGc                                            >  1:325171/1‑81 (MQ=255)                                  gCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGt                                         <  2:325140/81‑1 (MQ=255)                                  gCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGt                                         <  2:325142/81‑1 (MQ=255)                                  gCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGt                                         <  2:325141/81‑1 (MQ=255)                                          aCAACGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTcgcg                                 >  2:325169/1‑81 (MQ=255)                                             aCGTGTTTTATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCt                              >  2:325174/1‑81 (MQ=255)                                                    ttATCACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCg                        <  1:325143/80‑1 (MQ=255)                                                        cACGGGCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTtac                   >  2:325175/1‑81 (MQ=255)                                                            ggCAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGacgacg               <  1:325144/81‑1 (MQ=255)                                                              cAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGacgacg               >  1:325182/1‑79 (MQ=255)                                                              cAGAAAGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGaa             <  2:325145/81‑1 (MQ=255)                                                                 aaaGGCCATTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAagag          >  1:325173/1‑81 (MQ=255)                                                                        aTTATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAgc   <  2:325146/81‑1 (MQ=255)                                                                         ttATGCCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAgcg  >  1:325179/1‑81 (MQ=255)                                                                              |                                                                            TGACGATATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCG  >  NC_000913/1832541‑1832693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑