Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,837	G→A	L151L (CTG→CTA)	cdgI →	putative c‑di‑GMP binding protein CdgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,837	0	G	A	100.0%	43.7 / NA	13	L151L (CTG→CTA)	cdgI	putative c‑di‑GMP binding protein CdgI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

CATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTT  >  NC_000913/1870764‑1870902                                                                          |                                                                  cATTTTTAAGTGGCCTTATCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTAATCCAGa                                                            >  1:330608/1‑81 (MQ=255) cATTTTTAAGTGGCCTTATCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTAATCCAGa                                                            >  2:330600/1‑81 (MQ=255)             gCCTTATCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAatg                                                <  1:330583/81‑1 (MQ=255)             gCCTTATCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAatg                                                <  1:330584/81‑1 (MQ=255)               cTTATCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAatgat                                              >  1:330601/1‑81 (MQ=255)                                     ttGTAATTATCCATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTcc                        <  1:330585/81‑1 (MQ=255)                                      tGTAATTATCCATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCg                       >  2:330607/1‑81 (MQ=255)                                           ttATCCATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAAc                  <  1:330586/81‑1 (MQ=255)                                            tATCCATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACt                 >  2:330613/1‑81 (MQ=255)                                                cATAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGt             <  1:330587/81‑1 (MQ=255)                                                 aTAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGtt            >  2:330609/1‑81 (MQ=255)                                                 aTAAACCAATTAACGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGtt            >  2:330610/1‑81 (MQ=255)                                                            aaCGGCAGTACACTAATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGttt  >  1:330611/1‑80 (MQ=255)                                                                          |                                                                  CATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTT  >  NC_000913/1870764‑1870902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑