Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	268,409	C→T	E184E (GAG→GAA)	ykfA ←	CP4‑6 prophage; putative GTP‑binding protein YkfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	268,409	0	C	T	100.0%	180.8 / NA	49	E184E (GAG→GAA)	ykfA	CP4‑6 prophage; putative GTP‑binding protein YkfA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/21);  total (28/21)

ATCCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGCTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGTTC  >  NC_000913/268332‑268486                                                                              |                                                                              atcCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTc                                                                             <  2:60658/80‑1 (MQ=255)   ccGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATg                                                                          >  2:60706/1‑81 (MQ=255)   ccGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATg                                                                          >  2:60722/1‑81 (MQ=255)    cGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATg                                                                          <  2:60659/80‑1 (MQ=255)          gCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAgcagg                                                                   >  1:60723/1‑81 (MQ=255)               cACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGAt                                                              >  2:386178/1‑81 (MQ=255)                aCCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGAt                                                              <  2:60660/80‑1 (MQ=255)                    tAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGc                                                         >  1:60712/1‑81 (MQ=255)                     aaGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCg                                                        >  2:60724/1‑81 (MQ=255)                     aaGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCg                                                        >  1:60709/1‑81 (MQ=255)                        cccccATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCt                                                     >  2:60710/1‑81 (MQ=255)                                 tAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttct                                             <  2:60661/80‑1 (MQ=255)                                   gACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttctgct                                          <  2:60662/81‑1 (MQ=255)                                      ggACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttctgcttct                                       >  1:60725/1‑81 (MQ=255)                                       gACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttctgcttctg                                      >  2:60719/1‑81 (MQ=255)                                         ccGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTgcg                                    >  2:60743/1‑81 (MQ=255)                                          cGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTgcgc                                   <  2:60664/81‑1 (MQ=255)                                          cGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTgcgc                                   <  2:60663/81‑1 (MQ=255)                                          cGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTgcgc                                   >  2:60734/1‑81 (MQ=255)                                           gACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGcc                                  >  1:60741/1‑81 (MQ=255)                                             cacaGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGt                                <  2:60665/81‑1 (MQ=255)                                              acaGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTg                               >  1:60728/1‑81 (MQ=255)                                               caGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTgg                              <  2:60667/81‑1 (MQ=255)                                               caGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTgg                              <  1:60666/81‑1 (MQ=255)                                               caGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTg                               >  1:60713/1‑80 (MQ=255)                                               caGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTg                               >  2:60742/1‑80 (MQ=255)                                                    cacacCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAAc                         >  2:60729/1‑81 (MQ=255)                                                    cacacCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAAc                         <  1:60668/81‑1 (MQ=255)                                                      cacCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTg                       >  1:60739/1‑81 (MQ=255)                                                       acCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGt                      <  2:745608/81‑1 (MQ=255)                                                       acCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGt                      >  1:60744/1‑81 (MQ=255)                                                        ccGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTc                     >  2:60738/1‑81 (MQ=255)                                                        ccGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTc                     <  2:60669/81‑1 (MQ=255)                                                          ggATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTccc                   >  1:60735/1‑81 (MQ=255)                                                             tGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATCCTGCTTCTGCGCCGTGGACAACTGTCCCCCa                >  2:60718/1‑81 (MQ=255)                                                              ggACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCAc               >  1:60737/1‑81 (MQ=255)                                                               gACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCAc               <  2:60670/80‑1 (MQ=255)                                                                  gggCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCt           >  2:60732/1‑81 (MQ=255)                                                                  gggCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCt           <  1:60671/81‑1 (MQ=255)                                                                  gggCTGGAACAGTTCATGCAGCAGGCAGATTTCGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCt           <  2:60672/81‑1 (MQ=255)                                                                   ggCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTg          <  1:60673/81‑1 (MQ=255)                                                                    gCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTgg         >  1:60717/1‑81 (MQ=255)                                                                    gCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTgg         >  1:60745/1‑81 (MQ=255)                                                                     cTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGt        <  1:60675/81‑1 (MQ=255)                                                                     cTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGt        <  1:60674/81‑1 (MQ=255)                                                                        gAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAgg     >  1:60753/1‑81 (MQ=255)                                                                        gAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAg      <  1:60676/80‑1 (MQ=255)                                                                         aaCAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGt    >  1:60727/1‑81 (MQ=255)                                                                           cAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGTTc  <  1:60677/81‑1 (MQ=255)                                                                              |                                                                              ATCCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGCTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGTTC  >  NC_000913/268332‑268486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑