Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,917,063	C→A	A269E (GCG→GAG)	yebS →	intermembrane transport protein YebS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,917,063	0	C	A	100.0%	78.3 / NA	22	A269E (GCG→GAG)	yebS	intermembrane transport protein YebS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/13);  total (9/13)

AGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACGGAGGACG  >  NC_000913/1916988‑1917138                                                                            |                                                                            aGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTg                                                                         <  1:337584/80‑1 (MQ=255) aGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGt                                                                        >  2:337615/1‑81 (MQ=255)   cGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTa                                                                      <  2:337585/81‑1 (MQ=255)    gTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAg                                                                     <  1:337586/81‑1 (MQ=255)    gTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAg                                                                     >  1:337614/1‑81 (MQ=255)              cccGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATc                                                             <  2:337589/79‑1 (MQ=255)              cccGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCg                                                            <  1:337587/80‑1 (MQ=255)              cccGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGt                                                           <  1:337588/81‑1 (MQ=255)                 gcgTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGACGGAGCTGTTAGCGTCAATCGttt                                                         >  2:337617/1‑80 (MQ=255)                   gTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTAt                                                      >  1:337624/1‑81 (MQ=255)                         atatCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTAc                                                <  2:337590/81‑1 (MQ=255)                          tatCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTAcc                                               <  1:337591/81‑1 (MQ=255)                               cGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCa                                          <  1:337592/81‑1 (MQ=255)                                    cGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCt                                      <  2:337593/80‑1 (MQ=255)                                          cGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGcc                                >  1:337616/1‑80 (MQ=255)                                           gTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTa                              >  1:337625/1‑81 (MQ=255)                                                cGTCATAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTc                          >  2:337606/1‑80 (MQ=255)                                                     tAGTCTGCAAAAATGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCAt                    <  1:337594/81‑1 (MQ=255)                                                               aaaTGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAAc          <  1:337595/81‑1 (MQ=255)                                                                aaTGCTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACg         >  1:337622/1‑81 (MQ=255)                                                                    cTGGGCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACggagg     <  2:337596/81‑1 (MQ=255)                                                                      gggCGGAGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACGGAGGAc   >  2:337627/1‑81 (MQ=255)                                                                       ggcggaGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACggagga    <  1:337598/79‑1 (MQ=255)                                                                       ggcggaGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACGGAGGACg  <  1:337597/81‑1 (MQ=255)                                                                            |                                                                            AGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGGCGCTGTTAGCGTCAATCGTTTTATTGTTACCTGCCAACCTGTTGCCTATTTCTATCATTTATCTGAACGGAGGACG  >  NC_000913/1916988‑1917138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑