Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,777	A→G	D21D (GAT→GAC)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,777	0	A	G	100.0%	102.5 / NA	29	D21D (GAT→GAC)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

GTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTC  >  NC_000913/1928702‑1928853                                                                            |                                                                             gTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGAt                                                                         <  2:339435/81‑1 (MQ=255)  tAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATg                                                                        <  2:339436/81‑1 (MQ=255)   aaCTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATgc                                                                       >  1:339467/1‑81 (MQ=255)      tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGcgcg                                                                    >  1:339471/1‑81 (MQ=255)        ttttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGt                                                                   <  1:339437/80‑1 (MQ=255)               gttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCa                                                            <  1:339438/80‑1 (MQ=255)                     ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCg                                                     >  1:339489/1‑81 (MQ=255)                      gTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGt                                                    <  1:339439/81‑1 (MQ=255)                         gTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCAt                                                 >  2:339480/1‑81 (MQ=255)                          tCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATg                                                <  1:339440/81‑1 (MQ=255)                              ggACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGt                                            >  1:339491/1‑81 (MQ=255)                               gACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTg                                           >  1:339468/1‑81 (MQ=255)                                aCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgg                                          >  2:339466/1‑81 (MQ=255)                                  ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTggg                                         >  2:339478/1‑80 (MQ=255)                                      ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATg                                    >  1:339485/1‑81 (MQ=255)                                        cTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcg                                  <  1:339441/81‑1 (MQ=255)                                             aaCGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcgg                             >  1:339476/1‑81 (MQ=255)                                              aCGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGggcggc                            >  2:339474/1‑81 (MQ=255)                                                 cgTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCttt                         <  2:339442/81‑1 (MQ=255)                                                     tCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGt                     <  2:339443/81‑1 (MQ=255)                                                          ccGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCAt                >  1:339486/1‑81 (MQ=255)                                                              aGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTa            <  2:339444/81‑1 (MQ=255)                                                                  agtagtAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGt        <  1:339445/81‑1 (MQ=255)                                                                   gtagtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGtt       <  2:339446/81‑1 (MQ=255)                                                                     agtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct     >  1:339482/1‑81 (MQ=255)                                                                      gtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct     <  2:339447/80‑1 (MQ=255)                                                                      gtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct     <  2:339449/80‑1 (MQ=255)                                                                      gtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGCGCGGCTTTTGGTAGCATGTTATTGttct     <  1:339448/80‑1 (MQ=255)                                                                        aaTTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttc  >  2:339479/1‑81 (MQ=255)                                                                            |                                                                             GTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTC  >  NC_000913/1928702‑1928853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑