Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,940,886	C→T	L250L (CTG→CTA)	mepM ←	peptidoglycan DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,940,886	0	C	T	100.0%	82.4 / NA	23	L250L (CTG→CTA)	mepM	peptidoglycan DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

GTTACGATCGTAGAATTTGCCATCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGCAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTCAG  >  NC_000913/1940808‑1940960                                                                               |                                                                           gTTACGATCGTAGAATTTGCCATCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCcagtag                                                                          <  2:341422/81‑1 (MQ=255)    aCGATCGTAGAATTTGCCATCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCt                                                                        <  1:341423/80‑1 (MQ=255)             gAATTTGCCATCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCt                                                               <  1:341424/80‑1 (MQ=255)               aTTTGCCATCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCAc                                                            <  1:341425/81‑1 (MQ=255)                 ttGCCATCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGt                                                          >  1:341459/1‑81 (MQ=255)                      aTCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTAcc                                                     <  2:341426/81‑1 (MQ=255)                           cAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCa                                                 <  1:341427/80‑1 (MQ=255)                            aGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAg                                               >  1:341458/1‑81 (MQ=255)                            aGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAg                                               <  2:341428/81‑1 (MQ=255)                              cgcgGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCa                                             >  2:341460/1‑81 (MQ=255)                                      gCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTtctc                                       >  2:341456/1‑79 (MQ=255)                                        gTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCgaga                                   <  2:341429/81‑1 (MQ=255)                                                    aCCTTCTGAACGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCg                        <  2:341430/80‑1 (MQ=255)                                                             aCGCAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATc              <  1:341431/81‑1 (MQ=255)                                                                cAAACGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGcc           >  1:341466/1‑81 (MQ=255)                                                                  aaCGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCtt         >  1:341464/1‑81 (MQ=255)                                                                   aCGTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCttt        <  2:341432/81‑1 (MQ=255)                                                                     gTACGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCttttt      >  1:341463/1‑81 (MQ=255)                                                                        cGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTc    <  2:341435/80‑1 (MQ=255)                                                                        cGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTCa   <  2:341433/81‑1 (MQ=255)                                                                        cGCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTCa   <  2:341434/81‑1 (MQ=255)                                                                         gCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTCAg  >  1:341470/1‑81 (MQ=255)                                                                         gCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTCAg  <  1:341436/81‑1 (MQ=255)                                                                         gCCCAGTAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTCAg  >  2:341465/1‑81 (MQ=255)                                                                               |                                                                           GTTACGATCGTAGAATTTGCCATCTTCAGCGCGGATAGCGTAATAATCTTTACCTTCTGAACGCAAACGTACGCCCAGCAGCTGGCTTTGCTCACGTTTACCATCAAGCATTTCTCGAGACATTAACACCGCAAATTCATCGCCTTTTTTCAG  >  NC_000913/1940808‑1940960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑