Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,956,318	C→A	E230D (GAG→GAT)	torZ ←	trimethylamine N‑oxide reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,956,318	0	C	A	100.0%	58.6 / NA	18	E230D (GAG→GAT)	torZ	trimethylamine N‑oxide reductase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/9);  total (9/9)

GAGCGGATAGGATCAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCT  >  NC_000913/1956247‑1956389                                                                        |                                                                        gAGCGGATAGGATCAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTa                                                                >  1:344066/1‑81 (MQ=255)  aGCGGATAGGATCAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTAc                                                               <  1:344033/81‑1 (MQ=255)   gCGGATAGGATCAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACt                                                              <  1:344034/81‑1 (MQ=255)             tCAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGc                                                     >  1:344073/1‑80 (MQ=255)             tCAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCa                                                    <  2:344035/81‑1 (MQ=255)              cAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCaa                                                   >  2:344060/1‑81 (MQ=255)                      aTCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAg                                           >  1:344062/1‑81 (MQ=255)                        cACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAgg                                          >  2:344070/1‑80 (MQ=255)                             gTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTAtt                                      <  1:344038/79‑1 (MQ=255)                             gTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTAtt                                      <  1:344039/79‑1 (MQ=255)                             gTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTAtt                                      <  2:344037/79‑1 (MQ=255)                             gTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCa                                    <  1:344040/81‑1 (MQ=255)                                tGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCg                                 >  1:344081/1‑81 (MQ=255)                                  ccAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCggg                               <  2:344041/81‑1 (MQ=255)                                          tttCAGCTGATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTcc                       >  2:344067/1‑81 (MQ=255)                                                  gATGGAAGTATTCCAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGca               >  2:344069/1‑81 (MQ=255)                                                              ccAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGc   >  2:344071/1‑81 (MQ=255)                                                               cAGCCCTTGATCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCt  <  1:344042/81‑1 (MQ=255)                                                                        |                                                                        GAGCGGATAGGATCAATGGCAATCACTGGTTTGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCT  >  NC_000913/1956247‑1956389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑