Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,958,219	C→A	intergenic (‑87/+222)	torY ← / ← micL	cytochrome c quinol dehydrogenase TorY/small regulatory RNA MicL‑S

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,958,219	0	C	A	100.0%	23.3 / NA	8	intergenic (‑87/+222)	torY/micL	cytochrome c quinol dehydrogenase TorY/small regulatory RNA MicL‑S
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/5);  total (3/5)

AAATTACTGGACACCGAAATCATTGTATAATTAAATATACAACGAATTAACCGCTTGCATAATTAGGCACAACACTGCCTGAAACAATCGATAAAGAATATGATTTATTACAATGTAATCATTAATTGCTAAGGAATAACCCAGTTGCT  >  NC_000913/1958147‑1958295                                                                         |                                                                             aaattaCTGGACACCGAAATCATTGTATAATTAAATATACAACGAATTAACCGCTTGCATAATTAGGCACAAAACTGCCt                                                                       <  1:344327/80‑1 (MQ=255) aaattaCTGGACACCGAAATCATTGTATAATTAAATATACAACGAATTAACCGCTTGCATAATTAGGCACAAAACTGCCt                                                                       >  2:344342/1‑80 (MQ=255)  aattaCTGGACACCGAAATCATTGTATAATTAAATATACAACGAATTAACCGCTTGCATAATTAGGCACAAAACTGCCTg                                                                      <  1:344328/80‑1 (MQ=255)      aCTGGACACCGAAATCATTGTATAATTAAATATACAACGAATTAACCGCTTGCATAATTAGGCACAAAACTGCCTGAAAc                                                                  <  1:344329/80‑1 (MQ=255)       cTGGACACCGAAATCATTGTATAATTAAATATACAACGAATCAACCGCTTGCATAATTAGGCACAAAACTGCCTGAAACa                                                                 <  1:344330/80‑1 (MQ=255)             acCGAAATCATTGTATAATTAAATATACAACGAATTAACCGCTTGCATAATTAGGCACAAAACTGCCTGAAACAATCGAt                                                           >  1:344340/1‑80 (MQ=255)                                                                  ggCACAAAACTGCCTGAAACAATCGATAAAGAATATGATTTATTACAATGTAATCATTAATTGCTAAGGAATAACCCAGtt     >  2:344343/1‑81 (MQ=255)                                                                  ggCACAAAACTGCCTGAAACAATCGATAAAGAATATGATTTATTACAATGTAATCATTAATTGCTAAGGAATAACCCAGt      <  1:344331/80‑1 (MQ=255)                                                                     acaaaaCTGCCTGAAACAATCGATAAAGAATATGATTTATTACAATGTAATCATTAATTGCTAAGGAATAACCCAGTTGc   >  1:344346/1‑80 (MQ=255)                                                                     acaaaaCTGCCTGAAACAATCGATAAAGAATATGATTTATTACAATGTAATCATTAATTGCTAAGGAATAACCCAGTTGCt  <  1:344332/81‑1 (MQ=255)                                                                         |                                                                             AAATTACTGGACACCGAAATCATTGTATAATTAAATATACAACGAATTAACCGCTTGCATAATTAGGCACAACACTGCCTGAAACAATCGATAAAGAATATGATTTATTACAATGTAATCATTAATTGCTAAGGAATAACCCAGTTGCT  >  NC_000913/1958147‑1958295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑