Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,966,912	G→T	A42A (GCC→GCA)	cheZ ←	chemotaxis protein CheZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,966,912	0	G	T	100.0%	91.5 / NA	26	A42A (GCC→GCA)	cheZ	chemotaxis protein CheZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/12);  total (14/12)

CGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCGGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGA  >  NC_000913/1966835‑1966988                                                                              |                                                                             cGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGc                                                                            >  1:345829/1‑80 (MQ=255)   cTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAAt                                                                         <  2:345791/81‑1 (MQ=255)    tCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATg                                                                        >  1:345823/1‑81 (MQ=255)        cAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCt                                                                    <  2:345792/81‑1 (MQ=255)          gCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGa                                                                  >  1:345824/1‑81 (MQ=255)           ccTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGAt                                                                 >  2:345822/1‑81 (MQ=255)                  ggTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGccc                                                          <  2:345793/81‑1 (MQ=255)                    tCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCa                                                        <  1:345794/81‑1 (MQ=255)                      aTCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAg                                                       <  2:345795/80‑1 (MQ=255)                            acaacaTAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGc                                                <  2:345796/81‑1 (MQ=255)                             caacaTAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGc                                                >  2:345817/1‑80 (MQ=255)                                            gATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCgcagca                                <  1:345797/81‑1 (MQ=255)                                                gcgcgcATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATAcg                            >  1:345857/1‑81 (MQ=255)                                                 cgcgcATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATAcgc                           <  2:345798/81‑1 (MQ=255)                                                    gcATCGGGGATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGt                         >  2:345825/1‑80 (MQ=255)                                                         ggggATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCt                   <  2:345799/81‑1 (MQ=255)                                                         ggggATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCt                   >  1:345828/1‑81 (MQ=255)                                                            gATGGCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGcc                <  2:345800/81‑1 (MQ=255)                                                                gCTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATg            >  1:345833/1‑81 (MQ=255)                                                                 cTTCCGCCGCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATgc           >  1:345840/1‑81 (MQ=255)                                                                    ccgccgCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTTCCGATgcgcg        >  2:345830/1‑81 (MQ=255)                                                                    ccgccgCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATgcgcg        >  2:345820/1‑81 (MQ=255)                                                                    ccgccgCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATgcgcg        >  1:345826/1‑81 (MQ=255)                                                                    ccgccgCTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATgcgc         <  2:345801/80‑1 (MQ=255)                                                                      gccgcTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATgcgcgc       >  2:345837/1‑80 (MQ=255)                                                                          cTTCTGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGa  <  2:345802/81‑1 (MQ=255)                                                                              |                                                                             CGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCGGCAATGGCCTGATCCAGCCCCAGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGA  >  NC_000913/1966835‑1966988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑