Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,974,402	C→T	G308E (GGG→GAG)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,974,402	0	C	T	100.0%	79.8 / NA	23	G308E (GGG→GAG)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

CATCGGCATCATGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCCCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGAT  >  NC_000913/1974338‑1974478                                                                 |                                                                             cATCGGCATCATGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCa                                                               >  1:347132/1‑80 (MQ=255)  aTCGGCATCATGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCaaa                                                             >  2:347142/1‑81 (MQ=255)  aTCGGCATCATGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCaaa                                                             >  1:347149/1‑81 (MQ=255)       catcatGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCAcc                                                        <  1:347105/81‑1 (MQ=255)       catcatGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCAcc                                                        <  2:347106/81‑1 (MQ=255)       catcatGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCAcc                                                        <  2:347104/81‑1 (MQ=255)          catGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCAt                                                      >  2:347146/1‑80 (MQ=255)              cGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGAtt                                                  >  2:347137/1‑80 (MQ=255)                aaTCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAAc                                               <  1:347107/81‑1 (MQ=255)                       aTCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTc                                         >  1:347139/1‑80 (MQ=255)                                tCCTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTg                               >  1:347141/1‑81 (MQ=255)                                 ccTGCAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTg                               >  2:347145/1‑80 (MQ=255)                                     cAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGaa                            <  2:347109/79‑1 (MQ=255)                                     cAAATCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAAc                           <  2:347108/80‑1 (MQ=255)                                        aTCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTg                       >  1:347144/1‑81 (MQ=255)                                        aTCACGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTg                       <  1:347110/81‑1 (MQ=255)                                           accggCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGc                    <  2:347111/78‑1 (MQ=255)                                           aCGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTggg                     >  1:347147/1‑80 (MQ=255)                                            cGGGCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCa                   <  2:347112/81‑1 (MQ=255)                                              ggCGTTACGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAg                 <  1:347113/81‑1 (MQ=255)                                                    aCGTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGa           >  1:347148/1‑81 (MQ=255)                                                      gTTGTAACTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTTGCTGGAACACTGGGCAAGCATGGACt         >  1:347152/1‑81 (MQ=255)                                                             cTGCTCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGAt  <  2:347114/81‑1 (MQ=255)                                                                 |                                                                             CATCGGCATCATGCGAATCGACATCACTGATTCCTGCAAATCACGGGCGTTACGTTGTAACTGCCCCATGCTGGTTATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGAT  >  NC_000913/1974338‑1974478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑