Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,376	G→A	W196* (TGG→TGA)	yagH →	CP4‑6 prophage; putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,376	0	G	A	100.0%	166.0 / NA	46	W196* (TGG→TGA)	yagH	CP4‑6 prophage; putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

GCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGG  >  NC_000913/287298‑287453                                                                               |                                                                              gCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGAta                                                                             >  1:65270/1‑81 (MQ=255)   ccGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGAtac                                                                            >  1:65287/1‑80 (MQ=255)     gAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGAtactac                                                                         >  2:65279/1‑81 (MQ=255)         gcAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGa                                                                     >  1:65282/1‑81 (MQ=255)          cAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGAt                                                                    <  2:65223/81‑1 (MQ=255)          cAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGAt                                                                    <  2:65224/81‑1 (MQ=255)           aaaaCGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATg                                                                   >  1:65267/1‑81 (MQ=255)              aCGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGgcc                                                                <  2:65225/81‑1 (MQ=255)               cGCTGTTTACCGGCACGCCGCACTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGgccg                                                               <  2:65226/81‑1 (MQ=255)                gCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGgccgc                                                              <  1:65227/81‑1 (MQ=255)                 cTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGgccgcc                                                             <  1:65228/81‑1 (MQ=255)                  tGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGgccgcc                                                             >  2:65280/1‑80 (MQ=255)                    tttACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGccgccg                                                            <  1:65229/79‑1 (MQ=255)                    tttACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAg                                                          <  1:65230/81‑1 (MQ=255)                        ccGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGggc                                                       <  1:65231/80‑1 (MQ=255)                          ggCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGggcgg                                                     >  1:65292/1‑80 (MQ=255)                             aCGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCAcc                                                 <  1:65232/81‑1 (MQ=255)                              cgccgcTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCAc                                                  >  1:65277/1‑79 (MQ=255)                               gccgcTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAg                                               >  2:65298/1‑81 (MQ=255)                                ccgcTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGc                                              >  1:65288/1‑81 (MQ=255)                                 cgcTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCt                                             <  2:65233/81‑1 (MQ=255)                                  gcTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTa                                            >  2:65285/1‑81 (MQ=255)                                  gcTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTa                                            <  2:65234/81‑1 (MQ=255)                                       gCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAg                                        >  1:65304/1‑80 (MQ=255)                                          acacCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACg                                    >  2:65283/1‑81 (MQ=255)                                           cacCGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGc                                   >  1:65281/1‑81 (MQ=255)                                             ccGAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcg                                 >  2:65296/1‑81 (MQ=255)                                               gAAGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcgtc                               >  1:65284/1‑81 (MQ=255)                                                 aGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcgtcgt                             <  1:65235/81‑1 (MQ=255)                                                 aGGCGCGCACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcgtcgt                             >  2:65286/1‑81 (MQ=255)                                                    tgcgcACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCgtggt                          <  2:65236/80‑1 (MQ=255)                                                     gcgcACCTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCgtggtg                         >  1:65289/1‑81 (MQ=255)                                                          ccTGTATCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCg                    <  2:65237/81‑1 (MQ=255)                                                               aTCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTcc                >  1:65294/1‑80 (MQ=255)                                                               aTCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTcc                >  2:65297/1‑80 (MQ=255)                                                                tCGCCACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCaa              <  2:65238/81‑1 (MQ=255)                                                                   ccACGCGGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCaaaaa           <  1:65239/81‑1 (MQ=255)                                                                       gcgGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATc       <  2:65240/81‑1 (MQ=255)                                                                       gcgGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATc       <  1:65241/81‑1 (MQ=255)                                                                        cgGGATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCg      <  2:65242/81‑1 (MQ=255)                                                                          ggATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGAc    <  2:65243/81‑1 (MQ=255)                                                                          ggATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGAc    <  1:65244/81‑1 (MQ=255)                                                                          ggATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGAc    <  1:65245/81‑1 (MQ=255)                                                                          ggATGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGAc    >  1:65307/1‑81 (MQ=255)                                                                            aTGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACgg  <  2:65246/81‑1 (MQ=255)                                                                            aTGATACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACgg  >  1:65290/1‑81 (MQ=255)                                                                               |                                                                              GCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGG  >  NC_000913/287298‑287453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑