| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,995,681 | T→C | Y9Y (TAT→TAC) | yecU → | protein YecU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,995,681 | 0 | T | C | 85.7% | 14.9 / ‑3.2 | 7 | Y9Y (TAT→TAC) | yecU | protein YecU |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (5/1); minor base A (0/1); total (5/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
CGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATG > NC_000913/1995604‑1995753 | cGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACAtt < 2:350340/81‑1 (MQ=255) gAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACg > 2:350352/1‑81 (MQ=255) attaaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTAAATTAACGTATTTTATAGCACt < 1:350341/80‑1 (MQ=255) aaCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGcc > 1:350351/1‑80 (MQ=255) aCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGa > 2:350355/1‑81 (MQ=255) aaaaTATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTAtttt > 1:350354/1‑79 (MQ=255) ggTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATg > 2:350356/1‑81 (MQ=255) | CGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATG > NC_000913/1995604‑1995753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |