| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,015,409 | A→G | E177G (GAA→GGA) | fliG → | flagellar motor switch protein FliG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,015,409 | 0 | A | G | 96.5% | 98.5 / NA | 29 | E177G (GAA→GGA) | fliG | flagellar motor switch protein FliG |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (15/13); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTA > NC_000913/2015332‑2015486 | aCGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGt < 1:353374/81‑1 (MQ=255) cGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTa < 2:353375/81‑1 (MQ=255) gTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTAc > 1:353423/1‑81 (MQ=255) cgcCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAAt > 2:353415/1‑81 (MQ=255) gcCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATg > 1:353435/1‑81 (MQ=255) cgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCt > 1:353437/1‑81 (MQ=255) tGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGt > 2:353434/1‑80 (MQ=255) cGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAg < 2:353376/81‑1 (MQ=255) gTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGa < 2:353378/81‑1 (MQ=255) gTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGa < 2:353377/81‑1 (MQ=255) aTCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAAt > 1:353421/1‑81 (MQ=255) gCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAAtctc < 1:353379/81‑1 (MQ=255) aCCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAg > 2:353419/1‑81 (MQ=255) tGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCgcag > 1:353420/1‑81 (MQ=255) ggcgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCaaa < 1:353380/81‑1 (MQ=255) ggcgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCAACGGTCAGAATCTCAAGCGCAGCaa > 1:353427/1‑80 (MQ=255) gTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATg > 2:353418/1‑81 (MQ=255) cagccagcCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATggg < 1:353381/80‑1 (MQ=255) cagccagcCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggc < 1:353382/81‑1 (MQ=255) agccagccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggcg > 2:353422/1‑81 (MQ=255) ccagccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggcggc < 1:353383/81‑1 (MQ=255) cagccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGgcggcg > 2:353433/1‑81 (MQ=255) tGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACgg > 1:353436/1‑81 (MQ=255) tGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACgg > 2:353430/1‑81 (MQ=255) gCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCa < 1:353384/81‑1 (MQ=255) ggAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGc > 1:353431/1‑81 (MQ=255) aGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCg < 2:353385/81‑1 (MQ=255) cTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGaa < 2:353386/81‑1 (MQ=255) ccGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTa < 1:353387/81‑1 (MQ=255) | ACGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTA > NC_000913/2015332‑2015486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |