Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,083,052	T→C	I99I (ATT→ATC)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,083,052	0	T	C	100.0%	91.8 / NA	26	I99I (ATT→ATC)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/16);  total (10/16)

AGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTAC  >  NC_000913/2082974‑2083127                                                                               |                                                                            aGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCt                                                                           <  2:364026/81‑1 (MQ=255)    aCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTggg                                                                        >  1:364058/1‑81 (MQ=255)         cGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTaca                                                                   <  2:364027/81‑1 (MQ=255)           aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTacaa                                                                  >  1:364056/1‑80 (MQ=255)               gagaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAAtg                                                             >  1:364068/1‑81 (MQ=255)                  aaaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGc                                                          <  1:364029/81‑1 (MQ=255)                  aaaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGc                                                          <  2:364028/81‑1 (MQ=255)                    aaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGt                                                        >  1:364065/1‑81 (MQ=255)                       gAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGATCTGTATCCTGGGCTACAACAATGTGCGTTTc                                                     >  2:364066/1‑81 (MQ=255)                              cgTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTcgacgacg                                              <  2:364030/81‑1 (MQ=255)                               gTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTcgacgacga                                             <  1:364031/81‑1 (MQ=255)                                  tGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAg                                           <  2:364032/80‑1 (MQ=255)                                   gccgccCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGt                                          <  2:364033/80‑1 (MQ=255)                                    ccgccCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                                         <  1:364034/80‑1 (MQ=255)                                         cGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                                    >  1:364063/1‑80 (MQ=255)                                                   cGCTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAttgtt                          >  2:364061/1‑80 (MQ=255)                                                     cTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                       >  1:364064/1‑81 (MQ=255)                                                     cTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                       <  2:364035/81‑1 (MQ=255)                                                     cTTTTTACCGTACCGAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                       <  2:364036/81‑1 (MQ=255)                                                          taccgtaccgAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa                  >  1:364062/1‑81 (MQ=255)                                                          taccgtaccgAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTa                   <  2:364037/80‑1 (MQ=255)                                                             cgtaccgAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAttt               >  2:364078/1‑81 (MQ=255)                                                              gtaccgAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc              <  1:364040/81‑1 (MQ=255)                                                              gtaccgAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc              <  2:364038/81‑1 (MQ=255)                                                              gtaccgAAGACCTGTATCCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc              <  2:364039/81‑1 (MQ=255)                                                                            tATCCTGGGCTACAACAATGTGCGTTTCGACAACAAAGTCACACGCAACATTTTTTATCATAATTTCTACGATCCTTAc  <  1:364041/79‑1 (MQ=255)                                                                               |                                                                            AGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTAC  >  NC_000913/2082974‑2083127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑