Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,109,113	T→C	I158V (ATT→GTT)	rfbX ←	polyisoprenol‑linked O‑antigen repeat unit flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,109,113	0	T	C	100.0%	34.8 / NA	11	I158V (ATT→GTT)	rfbX	polyisoprenol‑linked O‑antigen repeat unit flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/7);  total (4/7)

TTGACTGAATAAAACCGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCA  >  NC_000913/2109044‑2109179                                                                      |                                                                   ttGACTGAATAAAACCGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCgg                                                          >  1:367971/1‑80 (MQ=255)    aCTGAATAAAACCGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCGGGAt                                                       <  1:367950/80‑1 (MQ=255)           aaaaCCGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCGGGATAAAAtac                                                >  2:367965/1‑80 (MQ=255)               ccGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACtt                                           <  1:367951/81‑1 (MQ=255)                  gCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACTTa                                          >  2:367972/1‑79 (MQ=255)                      ttGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACTTAAAGTCa                                    <  2:367952/81‑1 (MQ=255)                         cTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACTTAAAGTCAGcc                                 <  1:367953/81‑1 (MQ=255)                                          ttCACAAAAATAAATGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTttttcttt                 <  2:367954/80‑1 (MQ=255)                                                     aaatGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAg     >  1:367970/1‑81 (MQ=255)                                                      aatGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGc    <  1:367955/81‑1 (MQ=255)                                                        tGTTAGAGGGATAACAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCa  <  1:367956/81‑1 (MQ=255)                                                                      |                                                                   TTGACTGAATAAAACCGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCA  >  NC_000913/2109044‑2109179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑