Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,136,915	T→C	Y110C (TAC→TGC)	wza ←	outer membrane polysaccharide export protein Wza

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,136,915	0	T	C	100.0%	91.1 / NA	26	Y110C (TAC→TGC)	wza	outer membrane polysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/13);  total (13/13)

ACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATAC  >  NC_000913/2136837‑2136992                                                                               |                                                                              aCGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCAc                                                                             >  2:372778/1‑81 (MQ=255)    tGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGa                                                                          <  2:372747/81‑1 (MQ=255)      gACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGAcc                                                                        <  2:372748/81‑1 (MQ=255)      gACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGAc                                                                         <  1:372749/80‑1 (MQ=255)              cGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCg                                                                >  1:372805/1‑81 (MQ=255)                  ataCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCgtggt                                                            >  1:372796/1‑81 (MQ=255)                   taCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCgtggt                                                            <  2:372750/80‑1 (MQ=255)                        gTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTc                                                      >  1:372791/1‑81 (MQ=255)                          aaaaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCg                                                    >  1:372793/1‑81 (MQ=255)                           aaaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCgg                                                   >  2:372790/1‑81 (MQ=255)                             aCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGt                                                 <  1:372751/81‑1 (MQ=255)                                 aGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATc                                             >  2:372792/1‑81 (MQ=255)                                     ccGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATcc                                            >  2:372774/1‑78 (MQ=255)                                      cGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGa                                        <  1:372752/81‑1 (MQ=255)                                      cGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGa                                        >  2:372788/1‑81 (MQ=255)                                               gTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACAt                               >  2:372794/1‑81 (MQ=255)                                                           cGGTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTccc                   >  1:372797/1‑81 (MQ=255)                                                            ggTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTcccc                  <  2:372754/81‑1 (MQ=255)                                                            ggTGTCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTcccc                  <  1:372753/81‑1 (MQ=255)                                                                tCGCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGc              <  2:372755/81‑1 (MQ=255)                                                                  gCTGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGccc            <  2:372756/81‑1 (MQ=255)                                                                    tGGAGCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGGACGTCCCCCGGCCCGa          >  2:372807/1‑81 (MQ=255)                                                                        gCTGCGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGAcgc       >  1:372804/1‑80 (MQ=255)                                                                            cGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATa   <  2:372758/80‑1 (MQ=255)                                                                            cGGCACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATAc  <  2:372759/81‑1 (MQ=255)                                                                            cGGCACTGACCGGCTGGAGTGGTGAGTTCCGAGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATAc  <  2:372757/81‑1 (MQ=255)                                                                               |                                                                              ACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATAC  >  NC_000913/2136837‑2136992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑