| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,168,592 | C→A | intergenic (‑286/‑120) | yegR ← / → yegS | uncharacterized protein YegR/lipid kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,168,592 | 0 | C | A | 92.9% | 40.0 / ‑2.5 | 14 | intergenic (‑286/‑120) | yegR/yegS | uncharacterized protein YegR/lipid kinase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (8/5); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
AGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCT > NC_000913/2168514‑2168668 | aGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAAACa > 2:378830/1‑81 (MQ=255) gTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAAACACt < 1:378814/81‑1 (MQ=255) ttttttGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAAACACTGAAtcctc < 2:378815/79‑1 (MQ=255) cGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcctcc < 1:378816/81‑1 (MQ=255) gTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTAtcctcc > 2:378827/1‑80 (MQ=255) aGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACt > 1:378833/1‑81 (MQ=255) aTTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACttt > 2:378835/1‑80 (MQ=255) cTGTCCCCATTGCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCt < 2:378817/81‑1 (MQ=255) cccATTGCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt > 1:378837/1‑81 (MQ=255) tGCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTc > 1:378844/1‑81 (MQ=255) gCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTc < 2:378818/80‑1 (MQ=255) gCAAGCTCCTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTc > 2:378885/1‑80 (MQ=255) cTGGTGGTAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCttt < 1:378819/79‑1 (MQ=255) gtAAACACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCt > 2:378839/1‑81 (MQ=255) | AGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCT > NC_000913/2168514‑2168668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |