Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,197,477	T→C	intergenic (+179/‑2802)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,197,477	0	T	C	100.0%	27.0 / NA	9	intergenic (+179/‑2802)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/4);  total (5/4)

TTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTACTGGGGCAAAAAAAATTACCCTCGCTTGAGTGGTTAAAAATAGTGGCGACTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACC  >  NC_000913/2197401‑2197552                                                                             |                                                                            ttAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTACTGGGGCAAAAAAAATCAccc                                                                         >  2:383536/1‑81 (MQ=255)            gTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTACTGGGGCAAAAAAAATCACCCTCGCTTGAGTg                                                              >  2:383538/1‑81 (MQ=255)                  aCCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTACTGGGGCAAAAAAAATCACCCTCGCTTGAGTGGTTaa                                                         >  2:383533/1‑80 (MQ=255)                      gAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTACTGGGGCAAAAAAAATCACCCTCGCTTGAGTGGTTaaaaa                                                      >  1:383540/1‑79 (MQ=255)                               gAAATTGCACATGAAATTGCCCAGCGTCTACTGGGGCAAAAAAAATCACCCTCGCTTGAGTGGTTAAAAATAGTGGCGACt                                           <  1:383514/81‑1 (MQ=255)                                                tGCCCAGCGTCTACTGGGGCAAAAAAAATCACCCTCGCTTGAGTGGTTAAAAATAGTGGCGACTGATGAGCACATTCTTGc                          >  1:383544/1‑81 (MQ=255)                                                 gCCCAGCGTCTACTGGGGCAAAAAAAATCACCCTCGCTTGAGTGGTTAAAAATAGTGGCGACTGATGAGCACATTCTTGc                          <  2:383515/80‑1 (MQ=255)                                                             cTGGGGCAAAAAAAATCACCCTCGCTTGAGTGGTTAAAAATAGTGGCGACTGATGAGCACATTCTTGCCTCATTAGaaaa              <  2:383516/80‑1 (MQ=255)                                                                        aaaaTCACCCTCGCTTGAGTGGTTAAAAATAGTGGCGACTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAAcc  <  2:383517/81‑1 (MQ=255)                                                                             |                                                                            TTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTACTGGGGCAAAAAAAATTACCCTCGCTTGAGTGGTTAAAAATAGTGGCGACTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACC  >  NC_000913/2197401‑2197552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑