Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,198,232	G→A	intergenic (+934/‑2047)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,198,232	0	G	A	100.0%	53.8 / NA	16	intergenic (+934/‑2047)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/9);  total (7/9)

AGTACTACCAACCTGTTGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCGAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACTAAGTGAATTTAAGGTGTTT  >  NC_000913/2198163‑2198297                                                                      |                                                                  aGTACTACCAACCTGTTGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGtt                                                        >  2:383653/1‑81 (MQ=255)  gTACTACCAACCTGTTGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGtt                                                        >  2:383649/1‑80 (MQ=255)       acCAACCTGTTGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCa                                                  >  1:383656/1‑81 (MQ=255)        ccAACCTGTTGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCAt                                                 <  2:383620/81‑1 (MQ=255)        ccAACCTGTTGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCAt                                                 >  2:383652/1‑81 (MQ=255)                ttGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTaaa                                          <  1:383621/80‑1 (MQ=255)                         aTGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGattat                                <  2:383622/81‑1 (MQ=255)                              ttttCAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAAc                           >  1:383651/1‑81 (MQ=255)                                  cAACGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCtt                        <  1:383623/80‑1 (MQ=255)                                   aaCGACGGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTAc                      >  2:383665/1‑81 (MQ=255)                                    acgacgGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACt                     <  1:383624/81‑1 (MQ=255)                                    acgacgGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACt                     <  2:383625/81‑1 (MQ=255)                                     cgacgGATCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACTa                    <  2:383626/81‑1 (MQ=255)                                           aTCGCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACTAAGTGaa              >  2:383654/1‑81 (MQ=255)                                              gCTGGTACATATATTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACTAAGTGAAttt           <  2:383627/81‑1 (MQ=255)                                                       tatatTAAAGCTCCCAAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACTAAGTGAATTTAAGGTGttt  <  1:383628/81‑1 (MQ=255)                                                                      |                                                                  AGTACTACCAACCTGTTGGCAAGGATGGGTTTTCAACGACGGATCGCTGGTACATATATTAAAGCTCCCGAAGCGGTAGTTGAGGCATGGCTAAATGAAGATTATTCAACCTTACTAAGTGAATTTAAGGTGTTT  >  NC_000913/2198163‑2198297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑