Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,221,701	T→C	M97V (ATG→GTG)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,221,701	0	T	C	100.0%	83.5 / NA	24	M97V (ATG→GTG)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

GGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATC  >  NC_000913/2221628‑2221777                                                                          |                                                                             gggAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTg                                                                        <  2:387911/80‑1 (MQ=255) gggAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGa                                                                       >  2:387958/1‑81 (MQ=255)   gAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATc                                                                     <  2:387912/81‑1 (MQ=255)            gcgcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGgc                                                            >  1:387956/1‑81 (MQ=255)             cgcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGgc                                                            <  1:387913/80‑1 (MQ=255)              gcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGgcgc                                                          >  1:387953/1‑81 (MQ=255)                 gACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGa                                                       >  1:387964/1‑81 (MQ=255)                     gTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATc                                                   <  2:387914/81‑1 (MQ=255)                          gcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGAc                                              >  2:387963/1‑81 (MQ=255)                          gcaCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGAc                                              >  1:387949/1‑81 (MQ=255)                                cgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTa                                        >  2:387965/1‑81 (MQ=255)                                    aGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGgt                                    >  2:387969/1‑81 (MQ=255)                                        aagaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTg                                <  1:387915/81‑1 (MQ=255)                                            aaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGaaaa                            <  1:387917/81‑1 (MQ=255)                                            aaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGaaaa                            <  1:387916/81‑1 (MQ=255)                                             aagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAAt                           >  2:387961/1‑81 (MQ=255)                                              agagGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATc                          >  2:387957/1‑81 (MQ=255)                                               gagGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCg                         >  2:387918/1‑81 (MQ=255)                                                   aaTTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGcccc                     <  2:387919/81‑1 (MQ=255)                                                     ttttCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCaa                   <  1:387920/81‑1 (MQ=255)                                                          aGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTg               <  1:387921/80‑1 (MQ=255)                                                            gcggcTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGAcc            >  1:387960/1‑81 (MQ=255)                                                               gcTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTc         <  1:387922/81‑1 (MQ=255)                                                                  tAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCttt      >  2:387962/1‑81 (MQ=255)                                                                       ccaccaCCTGATCCTGAATGGCGCGGATATCATGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGatc  <  1:387923/80‑1 (MQ=255)                                                                          |                                                                             GGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGATC  >  NC_000913/2221628‑2221777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑