Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,245,248	T→G	T508P (ACG→CCG)	cirA ←	ferric dihyroxybenzoylserine outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,245,248	0	T	G	100.0%	97.8 / NA	28	T508P (ACG→CCG)	cirA	ferric dihyroxybenzoylserine outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/10);  total (18/10)

GTTTCCACGCCCTGAATACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTT  >  NC_000913/2245172‑2245322                                                                             |                                                                           gTTTCCACGCCCTGAATACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCa                                                                        <  2:392102/81‑1 (MQ=255)     ccACGCCCTGAATACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAAc                                                                    >  1:392132/1‑81 (MQ=255)     ccACGCCCTGAATACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAAc                                                                    >  2:392150/1‑81 (MQ=255)      cACGCCCTGAATACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAAc                                                                    <  1:392103/80‑1 (MQ=255)          cccTGAATACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACcaac                                                                >  2:392151/1‑80 (MQ=255)               aaTACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGtt                                                          >  2:392129/1‑81 (MQ=255)                    gAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTgg                                                      >  1:392152/1‑80 (MQ=255)                    gAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTgg                                                      >  2:392140/1‑80 (MQ=255)                         ttGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGccc                                                >  2:392142/1‑81 (MQ=255)                           gTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCg                                               <  1:392104/80‑1 (MQ=255)                              aaCGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTg                                            >  2:392136/1‑80 (MQ=255)                                     tagtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGtt                                     >  1:392139/1‑80 (MQ=255)                                      agtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGa                                   >  1:392133/1‑81 (MQ=255)                                      agtagCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGa                                   >  2:392147/1‑81 (MQ=255)                                         agCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGt                                >  2:392161/1‑81 (MQ=255)                                          gCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGt                                >  1:392137/1‑80 (MQ=255)                                          gCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTc                               <  2:392105/81‑1 (MQ=255)                                            tAAATACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGt                                >  2:392155/1‑78 (MQ=255)                                             aaaTACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGa                            >  1:392160/1‑81 (MQ=255)                                               aTACCGGTATGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACg                          >  1:392149/1‑81 (MQ=255)                                                        tGCGCCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGgctga                 <  2:392106/81‑1 (MQ=255)                                                          cgcCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGgctgatg               <  2:392107/81‑1 (MQ=255)                                                          cgcCGTCCGTTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGgctgat                <  2:392108/80‑1 (MQ=255)                                                             cgtccgtTAGCGCCCGGCTCAAAACCAACAAAGATTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGgctgatgctg            <  1:392109/81‑1 (MQ=255)                                                               tccgtTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGgctgatgctgat          <  2:392110/81‑1 (MQ=255)                                                                  gtTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGGCTGATGCTGATAc        >  1:392163/1‑80 (MQ=255)                                                                  gtTAGCGCCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGGCTGATGCTGATAc        >  1:392154/1‑80 (MQ=255)                                                                       cgcCCGGCTCAAAACCAACAAAGTTTTGGTAGCCCGATGCAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCtt  <  1:392111/81‑1 (MQ=255)                                                                             |                                                                           GTTTCCACGCCCTGAATACGAGCTTTGTTAACGTTGTAGTAGCTAAATACCGGTATGCGCCGTCCGTTAGCGCCCGTCTCAAAACCAACAAAGTTTTGGTAGCCCGGTGCAGCGTTGACGTCAGACGTACGGCTGATGCTGATACGATCTT  >  NC_000913/2245172‑2245322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑