Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,754	C→T	R151Q (CGA→CAA)	ecpE ←	putative fimbrial chaperone EcpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,754	0	C	T	100.0%	107.0 / NA	30	R151Q (CGA→CAA)	ecpE	putative fimbrial chaperone EcpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

CATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGG  >  NC_000913/304682‑304831                                                                         |                                                                              cATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTAcc                                                                       <  1:68719/81‑1 (MQ=255)   tgtgTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtactac                                                                        >  1:68756/1‑78 (MQ=255)       ttGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAAt                                                                  <  2:68720/80‑1 (MQ=255)          ccAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTa                                                              >  2:68750/1‑81 (MQ=255)              tgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCa                                                          >  2:68761/1‑81 (MQ=255)                   cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                      <  1:68721/80‑1 (MQ=255)                   cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                      <  1:68722/80‑1 (MQ=255)                   cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                      >  1:68771/1‑80 (MQ=255)                           tCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCgg                                             >  2:68759/1‑81 (MQ=255)                            ccTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGc                                            >  1:68752/1‑81 (MQ=255)                             cTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCt                                           <  2:68724/81‑1 (MQ=255)                             cTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCt                                           <  1:68723/81‑1 (MQ=255)                                tCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCg                                        <  1:68725/81‑1 (MQ=255)                                   ccTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGc                                     <  2:68726/81‑1 (MQ=255)                                       gTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                                 >  1:68777/1‑81 (MQ=255)                                           aaGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcg                             >  2:68765/1‑81 (MQ=255)                                             ggACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgcc                           <  1:68727/81‑1 (MQ=255)                                             ggACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgcc                           <  2:68728/81‑1 (MQ=255)                                             ggACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgcc                           <  2:68729/81‑1 (MQ=255)                                              gACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgccg                          >  1:68772/1‑81 (MQ=255)                                                        aCTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGc                <  2:68730/81‑1 (MQ=255)                                                         cTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCt               >  1:68770/1‑81 (MQ=255)                                                         cTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCt               <  2:68731/81‑1 (MQ=255)                                                         cTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCt               <  2:68732/81‑1 (MQ=255)                                                           gAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc             >  2:68776/1‑81 (MQ=255)                                                           gAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc             >  2:68764/1‑81 (MQ=255)                                                             aCCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGt           >  1:68766/1‑81 (MQ=255)                                                               cTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt         >  1:68779/1‑81 (MQ=255)                                                                    gTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt    <  1:68733/81‑1 (MQ=255)                                                                      ggTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATgg  >  1:68763/1‑81 (MQ=255)                                                                         |                                                                              CATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGG  >  NC_000913/304682‑304831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑