Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,779	T→C	K211R (AAA→AGA)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,779	0	T	C	100.0%	132.1 / NA	37	K211R (AAA→AGA)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)

ATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACC  >  NC_000913/2260701‑2260855                                                                               |                                                                             aTGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTg                                                                            >  1:394944/1‑81 (MQ=255)           cGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGtt                                                                  <  2:394893/81‑1 (MQ=255)            gCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGtt                                                                  >  2:394947/1‑80 (MQ=255)            gCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGtt                                                                  >  2:394941/1‑80 (MQ=255)             cccGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCa                                                                >  2:394938/1‑81 (MQ=255)               cGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATa                                                              >  2:394955/1‑81 (MQ=255)                gTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATAc                                                             >  1:394939/1‑81 (MQ=255)                 tCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACg                                                            <  2:394894/81‑1 (MQ=255)                       aGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTg                                                      >  2:394949/1‑81 (MQ=255)                          gACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCtt                                                   >  2:394954/1‑81 (MQ=255)                          gACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCtt                                                   >  1:394942/1‑81 (MQ=255)                           aCGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCtt                                                   <  2:394896/80‑1 (MQ=255)                           aCGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTc                                                  <  1:394895/81‑1 (MQ=255)                           aCGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTc                                                  <  2:394897/81‑1 (MQ=255)                               tATGCGCCTGCACTCTCTTACTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGc                                               >  2:394926/1‑80 (MQ=255)                                  gcgcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAAcc                                           >  1:394928/1‑81 (MQ=255)                                   cgcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAAcc                                           <  1:394898/80‑1 (MQ=255)                                     ccTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCt                                        <  2:394899/81‑1 (MQ=255)                                           ctctctTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATcc                                  <  1:394900/81‑1 (MQ=255)                                            tctctTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATc                                   >  1:394953/1‑79 (MQ=255)                                                  tcttACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTc                             <  1:394901/79‑1 (MQ=255)                                                      aCTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTgcg                        <  2:394902/80‑1 (MQ=255)                                                      aCTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTgc                         <  2:394903/79‑1 (MQ=255)                                                       cTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGcgcg                      >  1:394940/1‑81 (MQ=255)                                                          tCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCgg                     <  1:394904/79‑1 (MQ=255)                                                           cAGTGGTCGCCGTTTCAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGttt                  >  2:394957/1‑81 (MQ=255)                                                            aGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGttt                  <  1:394906/80‑1 (MQ=255)                                                            aGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGttt                  <  1:394905/80‑1 (MQ=255)                                                                gTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTttctt              >  2:394952/1‑80 (MQ=255)                                                                   gCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgc          <  1:394907/81‑1 (MQ=255)                                                                   gCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgc          >  2:394965/1‑81 (MQ=255)                                                                     cGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcgc        <  1:394908/81‑1 (MQ=255)                                                                     cGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcgc        >  2:394958/1‑81 (MQ=255)                                                                      gTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGcc       <  2:394909/81‑1 (MQ=255)                                                                         tGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAg     <  1:394910/80‑1 (MQ=255)                                                                          gAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGAc   >  2:394950/1‑81 (MQ=255)                                                                           aGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGAcc  <  1:394911/81‑1 (MQ=255)                                                                               |                                                                             ATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACC  >  NC_000913/2260701‑2260855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑