Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,275,814	A→G	E181G (GAA→GGA)	yejE →	putative oligopeptide ABC transporter membrane subunit YejE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,275,814	0	A	G	100.0%	106.9 / NA	30	E181G (GAA→GGA)	yejE	putative oligopeptide ABC transporter membrane subunit YejE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/11);  total (19/11)

GATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTG  >  NC_000913/2275740‑2275890                                                                           |                                                                             gATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATg                                                                        >  1:397716/1‑81 (MQ=255)   tGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGt                                                                      >  1:397738/1‑81 (MQ=255)          gCTGGCGGGGGCGCTACAAGGCTATTCCGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGAt                                                               >  2:397726/1‑81 (MQ=255)           cTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGAt                                                               <  1:397690/80‑1 (MQ=255)              gCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGcc                                                            <  1:397691/80‑1 (MQ=255)              gCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCg                                                           <  1:397692/81‑1 (MQ=255)                gggggCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGAc                                                         <  1:397694/81‑1 (MQ=255)                gggggCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGAc                                                         <  2:397693/81‑1 (MQ=255)                 ggggCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACg                                                        >  2:397695/1‑81 (MQ=255)                   ggCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCt                                                      >  1:397719/1‑81 (MQ=255)                   ggCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCt                                                      >  2:397729/1‑81 (MQ=255)                    gcgcTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTg                                                     >  1:397721/1‑81 (MQ=255)                     cgcTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGt                                                    <  1:397696/81‑1 (MQ=255)                     cgcTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGt                                                    >  1:397730/1‑81 (MQ=255)                       cTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGttt                                                  <  1:397697/81‑1 (MQ=255)                          cAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGttttt                                                >  2:397725/1‑80 (MQ=255)                          cAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGtttt                                                 <  2:397698/79‑1 (MQ=255)                           aaGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGa                                              >  1:479252/1‑81 (MQ=255)                               cTATTTCGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGattat                                          <  2:397699/81‑1 (MQ=255)                                      ggcggTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACtt                                   >  1:397731/1‑81 (MQ=255)                                        cggTAAAGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTc                                 >  2:397734/1‑81 (MQ=255)                                            aaaGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGc                             >  1:397745/1‑81 (MQ=255)                                             aaGTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGcg                            >  1:397735/1‑81 (MQ=255)                                               gTCGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGcgtc                          <  2:397700/81‑1 (MQ=255)                                                 cGATCTCTGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGcgtcg                         >  2:397724/1‑80 (MQ=255)                                                       ctGGGGACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGcc                  >  1:397722/1‑81 (MQ=255)                                                         ggggACAACGCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCt                 >  1:397723/1‑80 (MQ=255)                                                                  gCTTTATTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCtggt       <  2:397701/81‑1 (MQ=255)                                                                       aTTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGgctg  >  1:397746/1‑81 (MQ=255)                                                                       aTTGGAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGgctg  >  1:397727/1‑81 (MQ=255)                                                                           |                                                                             GATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTCGATCTCTGGGGACAACGCTTTATTGAAGTATGGTCGGGGATGCCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTG  >  NC_000913/2275740‑2275890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑