Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,638	A→T	I447F (ATC→TTC)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,638	0	A	T	100.0%	104.6 / NA	29	I447F (ATC→TTC)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/15);  total (14/15)

GTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTG  >  NC_000913/2277561‑2277712                                                                              |                                                                           gTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCa                                                                         <  2:398024/81‑1 (MQ=255) gTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCa                                                                         >  1:398063/1‑81 (MQ=255)  ttATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCAt                                                                        <  2:398025/81‑1 (MQ=255)  ttATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCAt                                                                        <  1:398026/81‑1 (MQ=255)    aTCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATAc                                                                      >  1:398066/1‑81 (MQ=255)     tCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATAc                                                                      <  2:398027/80‑1 (MQ=255)        ggcggAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTtga                                                                  >  2:398068/1‑81 (MQ=255)        ggcggAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTtga                                                                  >  1:398070/1‑81 (MQ=255)                    tggtggTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGAcat                                                       >  1:398079/1‑80 (MQ=255)                      gtggtCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGAcatca                                                     <  1:398028/80‑1 (MQ=255)                               gACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACa                                           <  2:398029/81‑1 (MQ=255)                                 cAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACaaa                                         <  1:398030/81‑1 (MQ=255)                                      tATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGt                                    <  2:398031/81‑1 (MQ=255)                                        ttGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                   <  2:398032/80‑1 (MQ=255)                                          gCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACa                                 <  2:398033/80‑1 (MQ=255)                                           cGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAgg                               >  1:398076/1‑81 (MQ=255)                                            gATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgc                              >  1:398073/1‑81 (MQ=255)                                              ttGCCAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcg                             >  1:398083/1‑80 (MQ=255)                                                 ccAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCaaa                         >  2:398074/1‑81 (MQ=255)                                                 ccAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCaa                          <  2:398034/80‑1 (MQ=255)                                                  cAGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAat                        <  1:398035/81‑1 (MQ=255)                                                   aGGGCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAata                       <  1:398036/81‑1 (MQ=255)                                                    gggCATTAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGTGCAAatat                      <  1:398037/81‑1 (MQ=255)                                                          tAATTCTTAAGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGc                >  2:398071/1‑81 (MQ=255)                                                                  gaGCCCTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGaaa        >  2:398085/2‑81 (MQ=255)                                                                       cTCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCAtt   <  2:398038/81‑1 (MQ=255)                                                                        tCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTg  >  1:398081/1‑81 (MQ=255)                                                                        tCGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTg  >  1:398077/1‑81 (MQ=255)                                                                         cGCTGTTCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTg  >  2:398091/1‑80 (MQ=255)                                                                              |                                                                           GTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTG  >  NC_000913/2277561‑2277712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑