Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,336,486	G→T	I53I (ATC→ATA)	yfaA ←	DUF2138 domain‑containing protein YfaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,336,486	0	G	T	100.0%	115.0 / NA	32	I53I (ATC→ATA)	yfaA	DUF2138 domain‑containing protein YfaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/15);  total (17/15)

GAATGGTGAGGAGATCTTTGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGA  >  NC_000913/2336413‑2336562                                                                          |                                                                             gAATGGTGAGGAGATCTTTGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTg                                                                         >  2:409429/1‑79 (MQ=255)     ggTGAGGAGATCTTTGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGAtt                                                                   >  2:409420/1‑81 (MQ=255)       tGAGGAGATCTTTGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGAttgt                                                                 >  1:409421/1‑81 (MQ=255)        gaggagATCTTTGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGAttgtt                                                                >  2:409415/1‑81 (MQ=255)            agaTCTTTGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGc                                                            <  1:409381/81‑1 (MQ=255)                   tGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGcacca                                                        <  2:409382/78‑1 (MQ=255)                   tGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGcac                                                          <  1:409383/76‑1 (MQ=255)                     agAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAgc                                                   <  2:409384/80‑1 (MQ=255)                      gAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAgcg                                                  <  1:409385/81‑1 (MQ=255)                       aaGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAgcgg                                                 <  2:409386/81‑1 (MQ=255)                         gCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAgcggcg                                               <  2:409387/81‑1 (MQ=255)                             gCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGAc                                             <  1:409388/79‑1 (MQ=255)                              cTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAAc                                          <  2:409389/81‑1 (MQ=255)                                    gagTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGttttt                                    >  1:409414/1‑81 (MQ=255)                                         gCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTgg                               >  1:409437/1‑81 (MQ=255)                                         gCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTgg                               >  1:409423/1‑81 (MQ=255)                                         gCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTgg                               <  1:409391/81‑1 (MQ=255)                                         gCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTg                                <  1:409390/80‑1 (MQ=255)                                            gTCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGcc                             >  2:409428/1‑80 (MQ=255)                                             tCGAGAAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCa                           >  1:409419/1‑81 (MQ=255)                                               gagaAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACt                         <  1:409392/81‑1 (MQ=255)                                                agaAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTg                        >  1:409418/1‑81 (MQ=255)                                                 gaAAGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGc                       <  1:409393/81‑1 (MQ=255)                                                  aaaGAGGTCCGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGc                       >  1:409439/1‑80 (MQ=255)                                                          ccGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATAtt              >  1:409432/1‑81 (MQ=255)                                                           cGGCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATAtt              <  1:409394/80‑1 (MQ=255)                                                            ggCTCATTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTg             >  1:409442/1‑80 (MQ=255)                                                                 aTTGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAAc         <  2:409395/79‑1 (MQ=255)                                                                  ttGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGcc      >  1:409424/1‑81 (MQ=255)                                                                   tGAGATCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGcc      >  1:409450/1‑80 (MQ=255)                                                                      gaTCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGa  >  2:409427/1‑81 (MQ=255)                                                                      gaTCTATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGa  >  2:409433/1‑81 (MQ=255)                                                                          |                                                                             GAATGGTGAGGAGATCTTTGGGAAGCTGGCTCAGAGAGTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGA  >  NC_000913/2336413‑2336562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑