Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,340,017	C→T	P151S (CCA→TCA)	ubiG →	bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,340,017	0	C	T	100.0%	94.3 / NA	26	P151S (CCA→TCA)	ubiG	bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/19);  total (7/19)

TGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGG  >  NC_000913/2339940‑2340089                                                                              |                                                                         tggtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAg                                                                       <  1:410160/81‑1 (MQ=255) tggtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAg                                                                       <  2:410161/81‑1 (MQ=255) tggtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAg                                                                       <  1:410159/81‑1 (MQ=255)  ggtgACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAgg                                                                      >  1:410204/1‑81 (MQ=255)       ccTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGgcggcg                                                                 <  1:410162/81‑1 (MQ=255)            aTGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTc                                                            >  2:410206/1‑81 (MQ=255)             tGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCt                                                           <  2:410163/81‑1 (MQ=255)               gagaTGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCttt                                                         <  2:410164/81‑1 (MQ=255)                 gaTGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCttttt                                                       <  1:410165/81‑1 (MQ=255)                         aGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGacac                                               >  2:410210/1‑81 (MQ=255)                         aGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGacac                                               <  1:410166/81‑1 (MQ=255)                           cACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACt                                              >  1:410209/1‑80 (MQ=255)                                 cccGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGc                                       <  1:410167/81‑1 (MQ=255)                                  ccGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCa                                      <  2:410168/81‑1 (MQ=255)                                         gCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGc                                 >  1:410207/1‑79 (MQ=255)                                         gCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGc                                 >  2:410211/1‑79 (MQ=255)                                          cagtcagtGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAg                              <  2:410169/81‑1 (MQ=255)                                               agtGGTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATg                         <  2:410170/81‑1 (MQ=255)                                                   gTCAGAGCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCt                      <  2:410171/80‑1 (MQ=255)                                                      agagCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGAt                   <  1:410172/80‑1 (MQ=255)                                                      agagCCTGTGCGCAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATg                  >  1:410208/1‑81 (MQ=255)                                                                cgcAACTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggtt         <  2:410173/80‑1 (MQ=255)                                                                   aaCTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTg     <  2:410174/81‑1 (MQ=255)                                                                     cTGGTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCg   <  1:410175/81‑1 (MQ=255)                                                                        gTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCgg  <  2:410176/79‑1 (MQ=255)                                                                        gTGAAATCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCgg  <  1:410177/79‑1 (MQ=255)                                                                              |                                                                         TGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGG  >  NC_000913/2339940‑2340089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑