Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,362,611	C→T	T341T (ACG→ACA)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,362,611	0	C	T	100.0%	121.0 / NA	34	T341T (ACG→ACA)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

CGCACACTTCCTGACGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCC  >  NC_000913/2362535‑2362686                                                                             |                                                                            cgcACACTTCCTGACGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTgg                                                                         >  2:414607/1‑81 (MQ=255)    acacTTCCTGACGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGc                                                                        >  2:414615/1‑79 (MQ=255)          ccTGACGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAgcgc                                                                  <  1:414574/79‑1 (MQ=255)             gACGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGt                                                             >  2:414620/1‑81 (MQ=255)              aCGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGtt                                                            <  2:414576/81‑1 (MQ=255)              aCGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGt                                                             <  1:414575/80‑1 (MQ=255)               cGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGtt                                                            >  2:414624/1‑80 (MQ=255)                 gATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAACGCAAAGtt                                                            >  2:414639/1‑78 (MQ=255)                    aGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATg                                                      >  1:414616/1‑81 (MQ=255)                     gCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGc                                                     >  1:414612/1‑81 (MQ=255)                     gCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGc                                                     <  1:414577/81‑1 (MQ=255)                      ccAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCt                                                    <  1:414578/81‑1 (MQ=255)                       cAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTc                                                   <  1:414579/81‑1 (MQ=255)                             gTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTtctc                                             >  2:414627/1‑81 (MQ=255)                              tggcgcGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTtctc                                             <  1:414581/78‑1 (MQ=255)                              tAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTtct                                              <  1:414580/79‑1 (MQ=255)                                    gTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAAccc                                      >  2:414617/1‑81 (MQ=255)                                       gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCATTCTCGAAACCCGaa                                   <  2:414582/81‑1 (MQ=255)                                          cTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGaaa                                  >  2:414634/1‑79 (MQ=255)                                              aaCGCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCa                            <  2:414583/81‑1 (MQ=255)                                                 gCACACGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTg                         >  2:414604/1‑81 (MQ=255)                                                   acacGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGcc                       <  1:414584/81‑1 (MQ=255)                                                    cacGCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCa                      <  2:414585/81‑1 (MQ=255)                                                     acGCCGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAg                     >  2:414638/1‑81 (MQ=255)                                                       gCAGAGCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGcc                   <  1:414586/81‑1 (MQ=255)                                                         agagCGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCg                 >  2:414621/1‑81 (MQ=255)                                                             cGAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCaa             <  2:414587/81‑1 (MQ=255)                                                              gAAAGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCaa             >  2:414633/1‑80 (MQ=255)                                                               aaaGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCaaaa           >  2:414646/1‑81 (MQ=255)                                                                aaGTGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAAt          >  2:414631/1‑81 (MQ=255)                                                                   tGCCGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGt       >  1:414635/1‑81 (MQ=255)                                                                     ccGTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGtt      <  2:414588/80‑1 (MQ=255)                                                                       gTCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGc   >  1:414632/1‑81 (MQ=255)                                                                        tCTGGTGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGcc  >  2:414651/1‑81 (MQ=255)                                                                             |                                                                            CGCACACTTCCTGACGGATAGCCAGGCTGTAGCGCGTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCC  >  NC_000913/2362535‑2362686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑