Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,372,873	T→C	I106T (ATC→ACC)	arnE →	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,372,873	0	T	C	100.0%	74.6 / NA	22	I106T (ATC→ACC)	arnE	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/14);  total (8/14)

AGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGC  >  NC_000913/2372799‑2372948                                                                           |                                                                            aGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCgg                                                                       >  2:416436/1‑81 (MQ=255)          gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGgt                                                              >  2:416418/1‑81 (MQ=255)                cGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATg                                                        <  1:416389/81‑1 (MQ=255)                 gAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATgg                                                       >  2:416438/1‑81 (MQ=255)                 gAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATgg                                                       >  2:416428/1‑81 (MQ=255)                 gAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATgg                                                       <  2:416390/81‑1 (MQ=255)                    ccGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGcc                                                    <  2:416391/81‑1 (MQ=255)                         aTCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGAtg                                               >  1:416432/1‑81 (MQ=255)                               gcgTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGa                                         <  2:416392/81‑1 (MQ=255)                                 gTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGatt                                       <  2:416393/81‑1 (MQ=255)                                     cTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTc                                   <  2:416394/81‑1 (MQ=255)                                             gggTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCat                           <  1:416395/81‑1 (MQ=255)                                             gggTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCat                           <  2:416397/81‑1 (MQ=255)                                             gggTGGCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCa                            <  2:416396/80‑1 (MQ=255)                                                  gCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCattatt                       <  2:416399/80‑1 (MQ=255)                                                  gCGTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCattatt                       <  2:416398/80‑1 (MQ=255)                                                    gTTCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGcc                    <  1:416400/81‑1 (MQ=255)                                                      tCATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCt                   >  2:416439/1‑80 (MQ=255)                                                       cATTATTGGCGGCATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCt                   >  1:416437/1‑79 (MQ=255)                                                               gcggcATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCa         <  1:416401/81‑1 (MQ=255)                                                                cggcATTGTGACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCaa        <  2:416402/81‑1 (MQ=255)                                                                      tgtgACCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGc  >  2:416431/1‑81 (MQ=255)                                                                           |                                                                            AGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGC  >  NC_000913/2372799‑2372948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑