Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,393,746	A→T	F434Y (TTC→TAC)	nuoL ←	NADH:quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,393,746	0	A	T	100.0%	103.2 / NA	29	F434Y (TTC→TAC)	nuoL	NADH:quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/20);  total (9/20)

AGCACAATCAGCGGCAGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCAT  >  NC_000913/2393668‑2393821                                                                               |                                                                            agcaCAATCAGCGGCAGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGtaa                                                                           <  2:420195/81‑1 (MQ=255)      aaTCAGCGGCAGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCAt                                                                      <  1:420196/81‑1 (MQ=255)      aaTCAGCGGCAGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAAGTTGTTCTTTTCCGTGGAAGACGATGTAAATCAt                                                                      <  2:420197/81‑1 (MQ=255)           gCGGCAGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACgg                                                                  >  1:420227/1‑80 (MQ=255)               cAGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGgt                                                             <  2:420198/81‑1 (MQ=255)                aGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGgtg                                                            >  2:420250/1‑81 (MQ=255)                      tgAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGc                                                      <  1:420199/81‑1 (MQ=255)                         gTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAg                                                   >  2:420239/1‑81 (MQ=255)                           tACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGt                                                 >  1:420248/1‑81 (MQ=255)                            aCCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGt                                                 <  1:420200/80‑1 (MQ=255)                              cccTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCAt                                              <  2:420202/81‑1 (MQ=255)                              cccTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCAt                                              <  2:420201/81‑1 (MQ=255)                                cTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATaa                                            <  2:420203/81‑1 (MQ=255)                                cTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATa                                             >  2:420233/1‑80 (MQ=255)                                  ttCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAAc                                          >  1:420249/1‑81 (MQ=255)                                    cACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACg                                         >  2:420243/1‑80 (MQ=255)                                     aCGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGc                                        <  1:420204/80‑1 (MQ=255)                                       ggCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCAcc                                     <  2:420205/81‑1 (MQ=255)                                        gcgtgagcgtgaATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCg                                    <  2:420206/81‑1 (MQ=255)                                         cgtgagcgtgaATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGa                                   <  2:420207/81‑1 (MQ=255)                                           tgagcgtgaATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGAcc                                 <  2:420208/81‑1 (MQ=255)                                                gtgaATTTGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGAcc                            >  1:420235/1‑81 (MQ=255)                                                     tttGTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGcca                       <  1:420209/81‑1 (MQ=255)                                                        gTTCTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGccacc                     <  2:420210/80‑1 (MQ=255)                                                           cTTTTCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATc                  <  1:420211/80‑1 (MQ=255)                                                              ttCCGTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGAt              >  2:420244/1‑81 (MQ=255)                                                                  gTGGAAGACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGat          <  2:420212/81‑1 (MQ=255)                                                                        gACGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGAcc    <  1:420213/81‑1 (MQ=255)                                                                         aCGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCa   <  1:420214/81‑1 (MQ=255)                                                                          cGATGTAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCAt  <  1:420215/81‑1 (MQ=255)                                                                               |                                                                            AGCACAATCAGCGGCAGGCTGTGAGTTACCCCTTTCACGGCGTGAGCGTGAATTTGTTCTTTTCCGTGGAAGACGATGAAAATCATACGGAAGGTGTAGAGCGAGGTCATAAACGCACCGACCAGACCTGCCACCATCAGATTGATATGACCAT  >  NC_000913/2393668‑2393821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑