Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,254	G→A	D63D (GAC→GAT)	nuoH ←	NADH:quinone oxidoreductase subunit H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,254	0	G	A	100.0%	130.9 / NA	36	D63D (GAC→GAT)	nuoH	NADH:quinone oxidoreductase subunit H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/12);  total (24/12)

AATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGC  >  NC_000913/2397176‑2397330                                                                               |                                                                             aaTCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATc                                                                            <  2:421024/81‑1 (MQ=255)     atcGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGc                                                                         >  2:421066/1‑80 (MQ=255)      tcGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCaa                                                                       >  2:421053/1‑81 (MQ=255)        ggTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAAc                                                                      >  2:421083/1‑80 (MQ=255)         gTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGACCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAAc                                                                      >  1:421072/1‑79 (MQ=255)           gCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGc                                                                  >  1:421073/1‑81 (MQ=255)            ccAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCt                                                                 >  2:421071/1‑81 (MQ=255)              aGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTg                                                                >  2:421065/1‑80 (MQ=255)                ggTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAg                                                             <  1:421025/81‑1 (MQ=255)                 gTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGc                                                            >  2:421082/1‑81 (MQ=255)                       aTGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCg                                                      >  2:421064/1‑81 (MQ=255)                              gATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGc                                               >  2:421069/1‑81 (MQ=255)                                  cGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAac                                           <  2:421026/81‑1 (MQ=255)                                   gagaATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAaca                                          <  1:421027/81‑1 (MQ=255)                                        tttCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGtt                                     <  1:421028/81‑1 (MQ=255)                                         ttCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGt                                      >  2:421067/1‑79 (MQ=255)                                          tCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTa                                    <  1:421029/80‑1 (MQ=255)                                          tCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAg                                   >  2:421074/1‑81 (MQ=255)                                             ggATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTc                                >  1:421095/1‑81 (MQ=255)                                              gATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTcc                               <  1:421030/81‑1 (MQ=255)                                              gATCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTcc                               <  2:421031/81‑1 (MQ=255)                                                tCCAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCg                              >  2:421088/1‑80 (MQ=255)                                                  cAGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTa                            <  2:421032/80‑1 (MQ=255)                                                   aGTCTTCTTTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                          <  2:421033/81‑1 (MQ=255)                                                       ttcttTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGtt                      >  1:421092/1‑81 (MQ=255)                                                       ttcttTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGtt                      >  2:421084/1‑81 (MQ=255)                                                        tcttTAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTc                     >  2:421098/1‑81 (MQ=255)                                                            tAAAGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCt                    <  1:421034/78‑1 (MQ=255)                                                             aaaGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGaa                >  2:421093/1‑81 (MQ=255)                                                             aaaGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGaa                >  2:421102/1‑81 (MQ=255)                                                              aaGAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGaa                >  1:421086/1‑80 (MQ=255)                                                                gAACATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGaa                >  1:421097/1‑78 (MQ=255)                                                                 aaCATTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGa            >  2:421087/1‑81 (MQ=255)                                                                    aTTTTGATCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAcc          >  1:421094/1‑80 (MQ=255)                                                                          aTCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAgg   >  1:421080/1‑81 (MQ=255)                                                                           tCATATCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGc  <  1:421035/81‑1 (MQ=255)                                                                               |                                                                             AATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGC  >  NC_000913/2397176‑2397330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑