Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	307,452	T→C	G627G (GGA→GGG)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	307,452	0	T	C	100.0%	134.2 / NA	37	G627G (GGA→GGG)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

ACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCG  >  NC_000913/307374‑307524                                                                               |                                                                         aCCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTccc                                                                        >  2:69346/1‑81 (MQ=255) aCCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTccc                                                                        >  2:69345/1‑81 (MQ=255)  cccTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTccc                                                                        <  1:69294/80‑1 (MQ=255)    cTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCt                                                                     <  2:69295/81‑1 (MQ=255)         aGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCg                                                                 >  1:69365/1‑80 (MQ=255)            cGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAAc                                                             <  1:69296/81‑1 (MQ=255)            cGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAAc                                                             <  1:69297/81‑1 (MQ=255)             gACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAAc                                                             >  2:69357/1‑80 (MQ=255)             gACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAAc                                                             >  1:69349/1‑80 (MQ=255)             gACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACg                                                            <  2:69298/81‑1 (MQ=255)                gCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGc                                                         <  1:69299/81‑1 (MQ=255)                   gCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTc                                                      >  1:69361/1‑81 (MQ=255)                      aTTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGaa                                                   >  1:69351/1‑81 (MQ=255)                       ttGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAAt                                                  <  1:69301/81‑1 (MQ=255)                       ttGGCGGTCAAGTTGGAATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAAt                                                  <  1:69300/81‑1 (MQ=255)                        tGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAAtt                                                 >  1:69356/1‑81 (MQ=255)                          gCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATtg                                                <  2:69302/80‑1 (MQ=255)                            ggTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATtgtg                                              >  2:69377/1‑80 (MQ=255)                              tCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCAt                                           <  1:69303/81‑1 (MQ=255)                                aaGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATAc                                         >  1:69372/1‑81 (MQ=255)                                    tGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGccc                                     >  2:69370/1‑81 (MQ=255)                                     ggTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGccc                                     <  1:69304/80‑1 (MQ=255)                                      gTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCccg                                   <  2:69305/81‑1 (MQ=255)                                         ttGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCccgccg                                >  2:69353/1‑81 (MQ=255)                                         ttGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCccgcc                                 <  1:69306/80‑1 (MQ=255)                                            aCGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCt                              <  2:69307/80‑1 (MQ=255)                                               tAGCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAgg                          >  2:69350/1‑81 (MQ=255)                                                 gCCGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGt                        >  2:69348/1‑81 (MQ=255)                                                  ccGTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGtt                       <  1:69308/81‑1 (MQ=255)                                                    gTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGtttt                     >  2:69355/1‑81 (MQ=255)                                                    gTCCGCCGCGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGtttt                     >  2:69352/1‑81 (MQ=255)                                                        gccgcGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtca                 >  2:69383/1‑81 (MQ=255)                                                        gccgcGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtca                 <  1:69309/81‑1 (MQ=255)                                                        gccgcGCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCACCGCCGCTGAGGGTTTTGtca                 >  1:69360/1‑81 (MQ=255)                                                          cgcgCTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtcatc               <  1:69310/81‑1 (MQ=255)                                                           gcgcTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtcatca              >  1:69359/1‑81 (MQ=255)                                                             gcTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCAcc            >  1:69364/1‑81 (MQ=255)                                                              cTATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCg           <  1:69311/81‑1 (MQ=255)                                                               tATTGACGTTCAGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCgg          >  1:69381/1‑81 (MQ=255)                                                                          aGCGTCCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCg  >  2:69379/1‑78 (MQ=255)                                                                               |                                                                         ACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCG  >  NC_000913/307374‑307524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑