Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,410,843	A→G	Y170Y (TAT→TAC)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,410,843	0	A	G	100.0%	114.3 / NA	32	Y170Y (TAT→TAC)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)

ACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAAT  >  NC_000913/2410769‑2410917                                                                           |                                                                           aCTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGcc                                                                       <  2:423741/80‑1 (MQ=255) aCTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCt                                                                      >  1:423788/1‑81 (MQ=255)     aCATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTc                                                                    <  1:423742/79‑1 (MQ=255)     aCATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCAc                                                                  <  2:890265/81‑1 (MQ=255)       atatACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACgc                                                                <  1:423743/81‑1 (MQ=255)       atatACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACgc                                                                <  2:423744/81‑1 (MQ=255)          taCAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACgcagc                                                             <  1:423745/81‑1 (MQ=255)                aTACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCa                                                       >  2:423816/1‑81 (MQ=255)                aTACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCa                                                       >  2:423781/1‑81 (MQ=255)                   cccAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTg                                                    >  1:423787/1‑81 (MQ=255)                      agcaccagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGtt                                                  >  2:423780/1‑80 (MQ=255)                         accagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGAc                                               >  1:423783/1‑80 (MQ=255)                         accagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGAc                                               >  2:423785/1‑80 (MQ=255)                         accagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACt                                              <  1:423746/81‑1 (MQ=255)                           cagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTAc                                            <  2:423747/81‑1 (MQ=255)                            agcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTAc                                            >  2:423791/1‑80 (MQ=255)                                 gACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGtt                                      >  1:423786/1‑81 (MQ=255)                                 gACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGtt                                      <  2:423748/81‑1 (MQ=255)                                   ccAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTcg                                    <  2:423749/81‑1 (MQ=255)                                   ccAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTcg                                    <  2:423750/81‑1 (MQ=255)                                       gCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTcggcgg                                >  2:423789/1‑81 (MQ=255)                                           aTAGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTc                            >  1:423794/1‑81 (MQ=255)                                             aGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGc                          >  2:423799/1‑81 (MQ=255)                                             aGGTGTTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGc                          >  2:423804/1‑81 (MQ=255)                                                 gtTACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGccac                       >  1:423793/1‑80 (MQ=255)                                                  ttACGCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGccacc                      <  1:423751/80‑1 (MQ=255)                                                      gCTAAAGAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGt                 <  2:423752/81‑1 (MQ=255)                                                            gAAACTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTcatcat           >  1:423808/1‑81 (MQ=255)                                                             aaaCTGAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATg          >  2:423838/1‑81 (MQ=255)                                                                  gAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGc      >  2:423805/1‑80 (MQ=255)                                                                  gAAGCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGc      >  1:423792/1‑80 (MQ=255)                                                                     gCCGTGGTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAAt  <  1:423753/81‑1 (MQ=255)                                                                           |                                                                           ACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAAT  >  NC_000913/2410769‑2410917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑