Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,412,278	T→C	Y106C (TAT→TGT)	yfbU ←	UPF0304 family protein YfbU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,412,278	0	T	C	100.0%	106.1 / NA	30	Y106C (TAT→TGT)	yfbU	UPF0304 family protein YfbU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/9);  total (21/9)

GGCGTCTGGGCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGTTCATCGATGGATTGC  >  NC_000913/2412200‑2412349                                                                               |                                                                        ggCGTCTGGGCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACaa                                                                       >  2:424060/1‑81 (MQ=255)   cGTCTGGGCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAAcc                                                                     >  2:424061/1‑81 (MQ=255)    gTCTGGGCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAAcc                                                                     >  2:424040/1‑80 (MQ=255)    gTCTGGGCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAAcc                                                                     >  2:424047/1‑80 (MQ=255)      cTGGGCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAg                                                                  >  1:424052/1‑81 (MQ=255)         ggCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGt                                                                <  1:424007/80‑1 (MQ=255)           cGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAAc                                                             >  2:424041/1‑81 (MQ=255)                 aaCCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCtt                                                       >  1:424056/1‑81 (MQ=255)                   gcGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCtt                                                       >  1:424036/2‑79 (MQ=255)                    cGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAg                                                    <  1:424008/81‑1 (MQ=255)                           tCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTggcggc                                             >  1:424049/1‑81 (MQ=255)                            ccGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGgcggcg                                            >  1:424055/1‑81 (MQ=255)                            ccGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGgcggcg                                            >  2:424048/1‑81 (MQ=255)                               gCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCa                                         <  1:424009/81‑1 (MQ=255)                                cGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCCAGGTAACGTGCTTCAGTGGCGGCGTCaa                                        <  2:424010/81‑1 (MQ=255)                                 gTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCaaa                                       <  1:424011/81‑1 (MQ=255)                                      aaTGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGcc                                    >  2:424051/1‑79 (MQ=255)                                      aaTGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGcc                                    >  1:424062/1‑79 (MQ=255)                                          ggTATAGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAgg                                >  2:424046/1‑79 (MQ=255)                                               aGCGCCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGa                         >  2:424063/1‑81 (MQ=255)                                                gcgcCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGac                        <  1:424012/81‑1 (MQ=255)                                                gcgcCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGa                         <  1:424013/80‑1 (MQ=255)                                                 cgcCCTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGaca                       >  1:424050/1‑81 (MQ=255)                                                     cTTCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACacgac                   >  1:424064/1‑81 (MQ=255)                                                       tCCACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGt                 >  2:424054/1‑81 (MQ=255)                                                        ccACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGtt                >  1:424066/1‑81 (MQ=255)                                                        ccACATTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGtt                >  2:424058/1‑81 (MQ=255)                                                          acaTTAACCATAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGTTCa              <  1:424014/81‑1 (MQ=255)                                                                   aTAAAGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGTTCATCGATGGAt     >  1:424067/1‑81 (MQ=255)                                                                      aaGCGGACACAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGTTCATCGATGGATTGc  <  2:424015/81‑1 (MQ=255)                                                                               |                                                                        GGCGTCTGGGCGTTAAAACCGTGAGTTCCGGCGTCAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGCTTCAGTGGCGGCGTCAAAGCCGAGGAAGGTGACACGACGTTCATCGATGGATTGC  >  NC_000913/2412200‑2412349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑