Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,437,410	G→T	P148T (CCT→ACT)	pdxB ←	erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,437,410	0	G	T	100.0%	101.8 / NA	28	P148T (CCT→ACT)	pdxB	erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/15);  total (13/15)

AGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAAC  >  NC_000913/2437336‑2437482                                                                           |                                                                         aGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATc                                                                    <  1:429069/81‑1 (MQ=255) aGTCAGAATATCCGCGCGCTGGACTAACTCAACCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATc                                                                    <  2:429068/81‑1 (MQ=255)  gTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCg                                                                   <  2:429070/81‑1 (MQ=255)      gAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCa                                                                 >  2:429104/1‑79 (MQ=255)       aaTATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAg                                                              >  1:429116/1‑81 (MQ=255)             cgcgcgCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGt                                                        >  1:429103/1‑81 (MQ=255)                gcgcTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGtttt                                                     <  1:429071/81‑1 (MQ=255)                     ggACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATcc                                                >  2:429112/1‑81 (MQ=255)                     ggACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATcc                                                >  1:429120/1‑81 (MQ=255)                     ggACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATcc                                                >  1:429107/1‑81 (MQ=255)                      gACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATc                                                 <  1:429072/79‑1 (MQ=255)                          aaCTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAAc                                           >  2:429115/1‑81 (MQ=255)                              cATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCtt                                       >  1:429106/1‑81 (MQ=255)                                tCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTc                                      >  1:429111/1‑80 (MQ=255)                                      gagcgGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTcgc                               >  1:429113/1‑81 (MQ=255)                                      gagcgGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTcgc                               <  2:429073/81‑1 (MQ=255)                                       agcgGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTcgcg                              <  1:429074/81‑1 (MQ=255)                                         cgGAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGcc                            <  2:429075/81‑1 (MQ=255)                                          ggAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTTATCCCTAACGCTTCCAGTCGCGCCt                           <  2:429076/81‑1 (MQ=255)                                          ggAAATCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCt                           >  2:429118/1‑81 (MQ=255)                                              aTCACCCTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCaa                       >  1:429105/1‑81 (MQ=255)                                                    cTCATCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACg                 <  1:429077/81‑1 (MQ=255)                                                       aTCCCCACGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTcc              <  1:429078/81‑1 (MQ=255)                                                             aCGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGtt        <  1:429079/81‑1 (MQ=255)                                                              cGGTCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTa       >  1:429114/1‑81 (MQ=255)                                                                 tCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTTCAATCGACGTCCAACGTTACCa    <  1:429081/81‑1 (MQ=255)                                                                 tCGGCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCa    <  2:429080/81‑1 (MQ=255)                                                                   ggCGCGAGTCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAAc  <  1:429082/81‑1 (MQ=255)                                                                           |                                                                         AGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCAAAGTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAAC  >  NC_000913/2437336‑2437482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑