Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,440,619	G→A	A329A (GCC→GCT)	fabB ←	beta‑ketoacyl‑[acyl carrier protein] synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,440,619	0	G	A	100.0%	139.5 / NA	39	A329A (GCC→GCT)	fabB	beta‑ketoacyl‑[acyl carrier protein] synthase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/18);  total (21/18)

GATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCC  >  NC_000913/2440541‑2440693                                                                               |                                                                           gATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGc                                                                          >  1:429741/1‑81 (MQ=255)     aaGCCGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTg                                                                      >  2:429727/1‑81 (MQ=255)     aaGCCGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTg                                                                      >  1:429710/1‑81 (MQ=255)        ccGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGtt                                                                   >  1:429731/1‑81 (MQ=255)         cGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTg                                                                  >  2:429745/1‑81 (MQ=255)          gtgtTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGc                                                                 <  2:429694/81‑1 (MQ=255)            gtTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTg                                                                  >  2:429730/1‑78 (MQ=255)             ttCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAg                                                               <  1:429695/80‑1 (MQ=255)                cAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAAt                                                           <  1:429696/81‑1 (MQ=255)                 aGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATc                                                          <  1:429697/81‑1 (MQ=255)                  gCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCg                                                         <  2:429698/81‑1 (MQ=255)                   cATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGc                                                        <  2:429699/81‑1 (MQ=255)                    aTCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGcc                                                       >  1:429743/1‑81 (MQ=255)                     tCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCg                                                      >  1:429749/1‑81 (MQ=255)                       agcagAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCggg                                                    <  2:429701/81‑1 (MQ=255)                       agcagAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCgg                                                     <  2:429700/80‑1 (MQ=255)                        gcagAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGc                                                   <  2:429702/81‑1 (MQ=255)                          agagagTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGccc                                                 >  2:429750/1‑79 (MQ=255)                               gTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATc                                            >  2:429759/1‑81 (MQ=255)                                 aGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGc                                          >  2:429744/1‑81 (MQ=255)                                      gCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAac                                     <  1:429703/81‑1 (MQ=255)                                         tCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACt                                  >  2:429760/1‑81 (MQ=255)                                             gTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCAc                              >  1:429757/1‑81 (MQ=255)                                               aCGCCAGCAGCGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACg                             >  2:429747/1‑80 (MQ=255)                                                     gcagcGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCt                      >  2:429764/1‑81 (MQ=255)                                                     gcagcGCCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCt                      <  2:429704/81‑1 (MQ=255)                                                        gcgcCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGcc                   <  2:429705/81‑1 (MQ=255)                                                        gcgcCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGc                    >  1:429739/1‑80 (MQ=255)                                                         cgcCCAGAGAGTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGc                    >  1:429765/1‑79 (MQ=255)                                                              agagagTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGctct             <  2:429706/81‑1 (MQ=255)                                                                  agTGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCa         >  2:429763/1‑81 (MQ=255)                                                                    tGACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACg       <  2:429707/81‑1 (MQ=255)                                                                     gACCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTACCCCCGAACTCTTCACGGATAGCTGCCAGCTCTTTCAcct      >  2:429766/1‑79 (MQ=255)                                                                      aCCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTc     <  2:429710/81‑1 (MQ=255)                                                                      aCCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTc     <  2:429709/81‑1 (MQ=255)                                                                      aCCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTc     <  2:429708/81‑1 (MQ=255)                                                                      aCCGGTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTc     >  2:429753/1‑81 (MQ=255)                                                                         ggTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGcc  <  2:429711/81‑1 (MQ=255)                                                                         ggTCATAGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGc   >  1:429746/1‑80 (MQ=255)                                                                               |                                                                           GATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCC  >  NC_000913/2440541‑2440693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑