Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,448,094	G→A	P116L (CCG→CTG)	prmB ←	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,448,094	0	G	A	100.0%	129.1 / NA	35	P116L (CCG→CTG)	prmB	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

CCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCC  >  NC_000913/2448016‑2448171                                                                               |                                                                              ccAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGc                                                                             <  1:431310/81‑1 (MQ=255)     tACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAgc                                                                         <  1:431312/81‑1 (MQ=255)     tACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAgc                                                                         <  1:431313/81‑1 (MQ=255)     tACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAgc                                                                         <  1:431314/81‑1 (MQ=255)       cacaTATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAgcgc                                                                       <  2:431316/81‑1 (MQ=255)       cacaTATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAgcgc                                                                       <  2:431315/81‑1 (MQ=255)       cacaTATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAgcgc                                                                       >  1:431362/1‑81 (MQ=255)                 aaaTATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCa                                                                <  1:431317/78‑1 (MQ=255)                           cGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCAt                                                    <  1:431318/80‑1 (MQ=255)                               tgcttgctGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACg                                               >  2:431355/1‑81 (MQ=255)                                gcttgctGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACg                                               >  1:431369/1‑80 (MQ=255)                                 cttgctGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGt                                              <  1:431319/80‑1 (MQ=255)                                       gATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAAtt                                        >  2:900757/1‑80 (MQ=255)                                        aTAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCa                                      >  2:431370/1‑81 (MQ=255)                                         tAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCAt                                     >  2:431356/1‑81 (MQ=255)                                         tAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCAt                                     >  2:431354/1‑81 (MQ=255)                                           aGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATgg                                   <  1:431321/81‑1 (MQ=255)                                           aGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATg                                    <  1:431320/80‑1 (MQ=255)                                              ccGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCg                                >  1:431365/1‑81 (MQ=255)                                               cGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCg                                <  1:431322/80‑1 (MQ=255)                                               cGGCAAATTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGc                               <  1:431323/81‑1 (MQ=255)                                                    aaTTTATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAAc                          <  1:431324/81‑1 (MQ=255)                                                      tttATTGTTGATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCa                        <  2:431325/81‑1 (MQ=255)                                                          ttgttgATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGc                     <  1:431326/80‑1 (MQ=255)                                                          ttgttgATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCt                    >  1:431361/1‑81 (MQ=255)                                                            gttgATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCttt                  >  2:431368/1‑81 (MQ=255)                                                               gATCAGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTttgtt               >  2:431379/1‑81 (MQ=255)                                                                   aGTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTc           <  2:431327/81‑1 (MQ=255)                                                                    gTTCACCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGt            >  2:431359/1‑79 (MQ=255)                                                                        aCCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGt       <  2:431328/80‑1 (MQ=255)                                                                        aCCAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGt       >  1:431329/1‑80 (MQ=255)                                                                          cAATCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAg    >  2:431385/1‑81 (MQ=255)                                                                           aaTCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGc   >  1:431391/1‑81 (MQ=255)                                                                            aTCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGcc  <  1:431330/81‑1 (MQ=255)                                                                            aTCAGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGc   >  1:431377/1‑80 (MQ=255)                                                                               |                                                                              CCAGTACACATATCTAAAATATGCTGCGGTTGCTTGCTGATAAGTCCGGCAAATTTATTGTTGATCAGTTCACCAATCGGCGAGCGCGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCC  >  NC_000913/2448016‑2448171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑