Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,485,331	G→A	E320K (GAA→AAA)	evgS →	sensory histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,485,331	0	G	A	100.0%	40.1 / NA	12	E320K (GAA→AAA)	evgS	sensory histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

AAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCC  >  NC_000913/2485255‑2485407                                                                             |                                                                             aaaaCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATAaaaa                                                                          >  2:437358/1‑81 (MQ=255)  aaaCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATAAAAAt                                                                         >  1:437368/1‑81 (MQ=255)    aCAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATAAAAATgg                                                                       >  1:437372/1‑81 (MQ=255)                        ccAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATAAAAATGGCTCGGTTCGGGGCGTTATg                                                    <  1:437353/80‑1 (MQ=255)                                 aGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTc                                          <  1:437354/81‑1 (MQ=255)                                       tGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAata                                    >  2:437369/1‑81 (MQ=255)                                           aaaTCCTTACTCGCCCCCCTATTCTATGACGGATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAAtattat                                >  1:437371/1‑81 (MQ=255)                                             aTCCTTACTCGCCCCCCTATTCTATGACGGATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATAttatta                              >  1:437359/1‑81 (MQ=255)                                                                ttCTATGACGGATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTa             <  1:437355/79‑1 (MQ=255)                                                                    aTGACGGATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAAtttt       >  1:437374/1‑81 (MQ=255)                                                                     tGACGGATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAAtt         <  1:437356/78‑1 (MQ=255)                                                                          gATAAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTcc  >  1:437376/1‑80 (MQ=255)                                                                             |                                                                             AAAACAGTGGATTAAGCAGCATCCCAATTTAAAGGTGCTGGAAAATCCTTACTCGCCCCCCTATTCTATGACGGATGAAAATGGCTCGGTTCGGGGCGTTATGGGGGACATTCTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCC  >  NC_000913/2485255‑2485407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑