Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,532,368	C→T	intergenic (+144/‑41)	cysZ → / → cysK	sulfate:H(+) symporter/O‑acetylserine sulfhydrylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,532,368	0	C	T	100.0%	93.3 / NA	26	intergenic (+144/‑41)	cysZ/cysK	sulfate:H(+) symporter/O‑acetylserine sulfhydrylase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

CATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTAT  >  NC_000913/2532290‑2532443                                                                               |                                                                            cATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTa                                                                           <  2:445937/81‑1 (MQ=255)  aTCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTAc                                                                          >  1:445972/1‑81 (MQ=255)     ttGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCtg                                                                       >  1:445957/1‑81 (MQ=255)         aTGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGGGCGGGTATGTTACCtgttgt                                                                   <  1:445938/81‑1 (MQ=255)         aTGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCtgttg                                                                    >  2:445959/1‑80 (MQ=255)             cATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATcc                                                               >  2:445970/1‑81 (MQ=255)              attattTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATcc                                                               >  1:445971/1‑80 (MQ=255)                     cccTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCa                                                       >  2:445965/1‑81 (MQ=255)                      ccTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCa                                                       <  1:445939/80‑1 (MQ=255)                               tataGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTaa                                              <  1:445940/80‑1 (MQ=255)                                       tGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGc                                      <  2:445941/80‑1 (MQ=255)                                         cTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCAt                                   >  2:445977/1‑81 (MQ=255)                                         cTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCAt                                   >  1:445962/1‑81 (MQ=255)                                             aTCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAg                                >  2:445984/1‑80 (MQ=255)                                              tCCTTACTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGt                               >  2:445978/1‑80 (MQ=255)                                                   aCTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAtt                         >  2:445967/1‑81 (MQ=255)                                                   aCTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAt                          <  2:445943/80‑1 (MQ=255)                                                   aCTTCCGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGAt                          <  1:445942/80‑1 (MQ=255)                                                        cGCATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTg                     >  2:445973/1‑80 (MQ=255)                                                          cATATTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAg                  <  1:445944/81‑1 (MQ=255)                                                            tatTCTCTGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGAt                <  2:445945/81‑1 (MQ=255)                                                                ctctGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAAc             <  2:445946/80‑1 (MQ=255)                                                                ctctGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACt            >  1:445966/1‑81 (MQ=255)                                                                   tGAGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTc           <  2:445947/79‑1 (MQ=255)                                                                     aGCGGGTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTg       <  2:445948/81‑1 (MQ=255)                                                                          gTATGTTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTAt  >  1:445982/1‑81 (MQ=255)                                                                               |                                                                            CATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAGTAAGATTTTTGAAGATAACTCGCTGACTAT  >  NC_000913/2532290‑2532443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑