Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,551,120	T→A	N33Y (AAC→TAC)	yfeY ←	DUF1131 domain‑containing lipoprotein YfeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,551,120	0	T	A	100.0%	134.7 / NA	38	N33Y (AAC→TAC)	yfeY	DUF1131 domain‑containing lipoprotein YfeY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/27);  total (11/27)

CTTGTTCTTGCAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTTCCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCATAGCGCATAAC  >  NC_000913/2551042‑2551196                                                                               |                                                                             cTTGTTCTTGCAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTAcc                                                                            <  2:449746/81‑1 (MQ=255) cTTGTTCTTGCAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTAcc                                                                            <  2:449745/81‑1 (MQ=255)     ttCTTGCAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACgg                                                                        <  1:449748/81‑1 (MQ=255)     ttCTTGCAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACgg                                                                        <  1:449747/81‑1 (MQ=255)           cAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGc                                                                  <  2:449750/81‑1 (MQ=255)           cAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGc                                                                  >  2:449805/1‑81 (MQ=255)           cAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGc                                                                  <  2:449751/81‑1 (MQ=255)           cAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGc                                                                  <  2:449749/81‑1 (MQ=255)             gtggtgTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCgg                                                                <  2:449752/81‑1 (MQ=255)              tggtgTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGc                                                               <  2:449754/81‑1 (MQ=255)              tggtgTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGc                                                               <  2:449753/81‑1 (MQ=255)                  gtgGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGAc                                                           >  2:449801/1‑81 (MQ=255)                  gtgGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGAc                                                           >  1:449813/1‑81 (MQ=255)                   tgGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGAcc                                                          <  2:449755/81‑1 (MQ=255)                      aCGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGt                                                       <  1:449756/81‑1 (MQ=255)                        gCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGtt                                                      >  1:449797/1‑80 (MQ=255)                          cGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAAc                                                   <  2:449757/81‑1 (MQ=255)                           gTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACt                                                  >  1:449803/1‑81 (MQ=255)                           gTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACt                                                  <  2:449758/81‑1 (MQ=255)                              aaTTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTgac                                               >  1:449804/1‑81 (MQ=255)                                    ccaaCGCCCAGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATc                                         <  2:449759/78‑1 (MQ=255)                                         gCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTgg                                     >  2:449808/1‑80 (MQ=255)                                           ccTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGaa                                   >  2:449810/1‑80 (MQ=255)                                           ccTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAAc                                  <  2:449760/81‑1 (MQ=255)                                                  gctcACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGt                           <  2:449761/81‑1 (MQ=255)                                                   ctcACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTc                          <  1:449762/81‑1 (MQ=255)                                                   ctcACTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTc                          <  2:449763/81‑1 (MQ=255)                                                      aCTTTGGTGGACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAg                        <  2:449764/80‑1 (MQ=255)                                                            gtggACGACCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAAt                 <  1:449765/81‑1 (MQ=255)                                                                 cgacCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCa             >  1:449792/1‑80 (MQ=255)                                                                 cgacCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCAt            <  1:449766/81‑1 (MQ=255)                                                                 cgacCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCAt            <  1:449767/81‑1 (MQ=255)                                                                 cgacCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCAt            <  1:449768/81‑1 (MQ=255)                                                                  gacCCAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCa             >  1:449811/1‑79 (MQ=255)                                                                    cccAAACCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCATAgc         >  2:449770/1‑81 (MQ=255)                                                                    cccAAACCAGTACCACAGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAAAGGCATAgc         <  2:449769/81‑1 (MQ=255)                                                                       aaaCCAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCATAGCGCa      <  1:449770/81‑1 (MQ=255)                                                                           cAGTACCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCATAGCGCATAAc  <  1:449771/81‑1 (MQ=255)                                                                               |                                                                             CTTGTTCTTGCAGTGGTGTGGACGCCGTTAATTCACCCACGCCCTGCTCGCTCACTTTGGTGGACGACCCAAACCAGTTCCACGGGTTAGCGGCAGACCAGTTAACTGACGACATCGTGGAACAGCCGGTCAGCATCAATGGCATAGCGCATAAC  >  NC_000913/2551042‑2551196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑