Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,619,147	C→T	S338S (TCG→TCA)	uraA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,619,147	0	C	T	100.0%	131.7 / NA	37	S338S (TCG→TCA)	uraA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/14);  total (23/14)

TGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGG  >  NC_000913/2619070‑2619211                                                                              |                                                                 tgtgCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGa                                                                >  1:464874/1‑80 (MQ=255)   tgCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGaa                                                               <  2:464799/79‑1 (MQ=255)         gttgtAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAAcgccgc                                                        <  2:464800/80‑1 (MQ=255)         gttgtAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACgccgcc                                                       >  1:464867/1‑81 (MQ=255)          ttgtAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACgccgcc                                                       >  2:464846/1‑80 (MQ=255)            gtAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCAt                                                    >  1:464859/1‑81 (MQ=255)                  cACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCgg                                              >  1:464860/1‑81 (MQ=255)                    cTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGc                                             >  2:464863/1‑80 (MQ=255)                       tcgattcgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCaa                                           <  1:464801/79‑1 (MQ=255)                       tcgattcgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATg                                         >  2:464851/1‑81 (MQ=255)                        cgattcgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATgg                                        >  2:464868/1‑81 (MQ=255)                        cgattcgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATgg                                        >  1:464856/1‑81 (MQ=255)                             cgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGatca                                   >  2:464849/1‑81 (MQ=255)                              gatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGatcat                                  >  1:464869/1‑81 (MQ=255)                               atCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGatcatc                                 >  2:464864/1‑81 (MQ=255)                                tCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCt                                <  1:464802/81‑1 (MQ=255)                                  aaaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTg                               >  2:464861/1‑80 (MQ=255)                                    aaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGa                             >  1:464862/1‑80 (MQ=255)                                        cGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCg                        <  2:464803/81‑1 (MQ=255)                                           aTACCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCgg                       >  1:464857/1‑79 (MQ=255)                                             aCCGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGc                   >  2:464872/1‑81 (MQ=255)                                              ccGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGcc                  <  2:464804/81‑1 (MQ=255)                                              ccGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGcc                  <  1:464805/81‑1 (MQ=255)                                               cGGAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGc                   >  1:464854/1‑79 (MQ=255)                                                ggAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAg                >  2:464852/1‑81 (MQ=255)                                                 gAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGt               >  1:464877/1‑81 (MQ=255)                                                 gAAGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGt               <  1:464806/81‑1 (MQ=255)                                                  aaGCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGtt              >  2:464876/1‑81 (MQ=255)                                                    gCACCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTa            >  2:464858/1‑81 (MQ=255)                                                      aCCGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTAcc          <  1:464807/81‑1 (MQ=255)                                                       ccGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTAcc          >  2:464880/1‑80 (MQ=255)                                                        cGATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGa        <  2:464808/81‑1 (MQ=255)                                                         gATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGAc       <  1:464810/81‑1 (MQ=255)                                                         gATGACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGAc       <  2:464809/81‑1 (MQ=255)                                                            gACACCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGACg      >  1:464866/1‑79 (MQ=255)                                                             acacCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGACGCa    <  2:464811/80‑1 (MQ=255)                                                              cacCATAAAGCAGCAGTGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGACGCAgg  <  2:464812/81‑1 (MQ=255)                                                                              |                                                                 TGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGG  >  NC_000913/2619070‑2619211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑