Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,631,603	G→A	I311I (ATC→ATT)	guaA ←	GMP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,631,603	0	G	A	100.0%	107.3 / NA	31	I311I (ATC→ATT)	guaA	GMP synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/18);  total (13/18)

ATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTGCCGGTACGTGA  >  NC_000913/2631526‑2631678                                                                              |                                                                            aTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCaatg                                                                          <  2:467030/81‑1 (MQ=255)  tGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCaatga                                                                         <  1:467031/81‑1 (MQ=255)   ggTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCaatgat                                                                        >  1:467073/1‑81 (MQ=255)       cccTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGAtttt                                                                     <  1:467032/80‑1 (MQ=255)       cccTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGAtttt                                                                     <  1:467033/80‑1 (MQ=255)        ccTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTAc                                                                   <  2:467034/81‑1 (MQ=255)        ccTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTAc                                                                   <  1:467035/81‑1 (MQ=255)             gccagccaCTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGtttt                                                              >  2:467078/1‑81 (MQ=255)             gccagccaCTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGtttt                                                              <  2:467036/81‑1 (MQ=255)                agccaCTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCt                                                           >  2:467091/1‑81 (MQ=255)                  ccaCTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTc                                                         <  2:467037/81‑1 (MQ=255)                     cTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCgg                                                      <  1:467039/81‑1 (MQ=255)                     cTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCgg                                                      <  2:467038/81‑1 (MQ=255)                          cGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGt                                                 <  1:467040/81‑1 (MQ=255)                           gTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGtt                                                >  1:467087/1‑81 (MQ=255)                            tCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGttt                                               <  2:467041/81‑1 (MQ=255)                             cTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGtttt                                              >  2:467081/1‑81 (MQ=255)                                ccAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGtttt                                              <  1:467043/78‑1 (MQ=255)                                ccAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGtttt                                              <  2:467042/78‑1 (MQ=255)                                ccAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCgc                                           >  2:467100/1‑81 (MQ=255)                                       cAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGccagccag                                    >  2:467096/1‑81 (MQ=255)                                        aGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGccagccag                                    <  2:467044/80‑1 (MQ=255)                                        aGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGccagccag                                    <  1:467045/80‑1 (MQ=255)                                                ttcATCGAATACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAg                           >  1:467084/1‑81 (MQ=255)                                                       aaTACTTCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGa                    >  1:467092/1‑81 (MQ=255)                                                            ttCAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGAtcttc               >  1:467101/1‑81 (MQ=255)                                                              cAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGAtcttc               <  1:467046/79‑1 (MQ=255)                                                              cAACGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTg             >  1:467110/1‑81 (MQ=255)                                                                aCGAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTGcc           <  1:467047/81‑1 (MQ=255)                                                                  gAAAACGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTGCCgg         >  2:467082/1‑81 (MQ=255)                                                                     aaCGCGACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTGCCGGTAc      <  1:467048/81‑1 (MQ=255)                                                                      aCGCGACCAATGATTTTATGTTTTGCTTCCGGATCGCTTTCGCCAGCCAGCGCTGACAGGATGCGATCTTCCGCCGGTACg     >  2:467106/1‑81 (MQ=255)                                                                        gcgACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTGCCGGTACGt    >  1:467102/1‑80 (MQ=255)                                                                         cgACCAATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTGCCGGTACGTGa  <  1:467049/81‑1 (MQ=255)                                                                              |                                                                            ATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATCTTCTGCCGGTACGTGA  >  NC_000913/2631526‑2631678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑