Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,648,726	G→A	L1188F (CTT→TTT)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,648,726	0	G	A	100.0%	100.2 / NA	29	L1188F (CTT→TTT)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/19);  total (10/19)

CTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAA  >  NC_000913/2648651‑2648801                                                                            |                                                                            cTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGc                                                                        <  2:470949/81‑1 (MQ=255)   ttGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCg                                                                      >  1:471012/1‑81 (MQ=255)    tGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGcc                                                                       <  1:470950/79‑1 (MQ=255)        gCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACg                                                                  >  1:471006/1‑80 (MQ=255)         cccAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACgg                                                                 <  2:470952/80‑1 (MQ=255)         cccAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGa                                                                <  2:470951/81‑1 (MQ=255)          ccAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGa                                                                >  2:470989/1‑80 (MQ=255)           cAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATa                                                              >  1:471017/1‑81 (MQ=255)             aCGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACg                                                            <  2:470953/81‑1 (MQ=255)               gCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCt                                                          >  2:470997/1‑81 (MQ=255)                         gggCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAActct                                                 <  2:470954/80‑1 (MQ=255)                          ggCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCttt                                               <  1:470955/81‑1 (MQ=255)                                   ggTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAAc                                       <  1:470956/80‑1 (MQ=255)                                    gTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACg                                      <  1:470957/80‑1 (MQ=255)                                      ataAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGc                                   >  2:471020/1‑81 (MQ=255)                                       taAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGc                                   <  1:470959/80‑1 (MQ=255)                                       taAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCg                                  <  1:470958/81‑1 (MQ=255)                                        aaaGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGa                                 >  2:471011/1‑81 (MQ=255)                                             gACGGAAACAGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGtt                             >  1:471007/1‑80 (MQ=255)                                                      aGGCCGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCggtggt                   <  1:470960/81‑1 (MQ=255)                                                          cGCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGc               <  2:470961/81‑1 (MQ=255)                                                           gCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGcc              <  1:470963/81‑1 (MQ=255)                                                           gCTGGCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGcc              <  2:470962/81‑1 (MQ=255)                                                               gCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCt           <  1:470964/80‑1 (MQ=255)                                                               gCGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTc          <  1:470965/81‑1 (MQ=255)                                                                cGGTTTGCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTca         <  2:470966/81‑1 (MQ=255)                                                                      gCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTcaacaac    >  1:471015/1‑80 (MQ=255)                                                                      gCTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTcaacaac    >  2:471009/1‑80 (MQ=255)                                                                       cTCAAAACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTcaacaacaa  <  1:470967/81‑1 (MQ=255)                                                                            |                                                                            CTTTGATGCCCAACGCCTGCAGTTGGGCTGCGTTGGTATAAAGTGACGGAAACAGGCCGCTGGCGGTTTGCTCAAGACAGCCGTACGGATACGCTTTTAACTCTTTGATATAACGTGCGATGTTCAGCGGTGGTTTGCCGCTCAACAACAA  >  NC_000913/2648651‑2648801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑