Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,689,761	G→T	R446R (CGC→CGA)	glrK ←	sensory histidine kinase GlrK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,689,761	0	G	T	100.0%	90.9 / NA	26	R446R (CGC→CGA)	glrK	sensory histidine kinase GlrK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/9);  total (17/9)

GACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGGTGGCTTCCCTGAA  >  NC_000913/2689687‑2689837                                                                           |                                                                             gacgacGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATa                                                                        >  1:479735/1‑81 (MQ=255)   cgacgGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATAc                                                                       >  2:479738/1‑80 (MQ=255)        gTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATccc                                                                 <  2:479711/81‑1 (MQ=255)        gTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATccc                                                                 <  2:479709/81‑1 (MQ=255)        gTAAGTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATccc                                                                 <  2:479710/81‑1 (MQ=255)                    ggAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCtt                                                      >  1:479737/1‑80 (MQ=255)                    ggAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCtt                                                      >  1:479745/1‑80 (MQ=255)                         caaacGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATccc                                                <  1:479712/81‑1 (MQ=255)                            acGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGa                                             >  2:479759/1‑81 (MQ=255)                              gTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGAcc                                           >  1:479743/1‑81 (MQ=255)                                    cccGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGcc                                     >  2:479744/1‑81 (MQ=255)                                    cccGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGc                                      <  2:479713/80‑1 (MQ=255)                                      cGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCt                                   >  2:479739/1‑81 (MQ=255)                                            cgtcgACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCg                             >  2:479749/1‑81 (MQ=255)                                                gACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcccc                         >  2:479758/1‑81 (MQ=255)                                                gACCAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGccc                          <  2:479714/80‑1 (MQ=255)                                                  ccAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCtt                       <  1:479715/81‑1 (MQ=255)                                                   cAGATACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCttt                      >  1:479748/1‑81 (MQ=255)                                                      aTACAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCg                   >  2:479746/1‑81 (MQ=255)                                                        aCAGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCt                 >  1:479752/1‑81 (MQ=255)                                                          aGTTCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCtgg               >  1:479760/1‑81 (MQ=255)                                                             tCCCCTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCtggtgg            >  1:479740/1‑81 (MQ=255)                                                                ccTTGCATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGGTGGCtt         <  2:479716/81‑1 (MQ=255)                                                                     cATACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGGTGGCTTCCCTg    <  2:479717/81‑1 (MQ=255)                                                                      aTACGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGGTGGCTTCCCTg    >  1:479761/1‑80 (MQ=255)                                                                        aCGTCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGGTGGCTTCCCTGaa  >  1:479753/1‑80 (MQ=255)                                                                           |                                                                             GACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGGTGGCTTCCCTGAA  >  NC_000913/2689687‑2689837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑