Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,693,373	G→C	A724G (GCC→GGC)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,693,373	0	G	C	100.0%	151.8 / NA	43	A724G (GCC→GGC)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/18);  total (25/18)

AGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTC  >  NC_000913/2693303‑2693449                                                                       |                                                                             aGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGt                                                                    >  1:480587/1‑81 (MQ=255)  gggTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGtt                                                                   <  2:480533/81‑1 (MQ=255)     tGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAAc                                                                >  2:480580/1‑81 (MQ=255)      ggCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACg                                                               >  1:480582/1‑81 (MQ=255)         cTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCtt                                                            <  1:480534/81‑1 (MQ=255)           gccgccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCa                                                          >  1:480584/1‑81 (MQ=255)              gccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCacc                                                        >  1:480602/1‑80 (MQ=255)              gccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCacc                                                        >  2:480595/1‑80 (MQ=255)                    aTCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGcc                                                 <  1:480535/81‑1 (MQ=255)                        aGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTTCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGAc                                             >  1:480589/1‑81 (MQ=255)                        aGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGAc                                             <  2:480536/81‑1 (MQ=255)                        aGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGAc                                             <  2:480538/81‑1 (MQ=255)                        aGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGAc                                             <  1:480537/81‑1 (MQ=255)                        aGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGAc                                             >  1:480576/1‑81 (MQ=255)                           tGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACggg                                          >  1:480598/1‑81 (MQ=255)                           tGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACggg                                          >  1:480588/1‑81 (MQ=255)                            ggCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACggg                                          >  1:480593/1‑80 (MQ=255)                             gCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGc                                         <  1:480539/80‑1 (MQ=255)                              cGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCag                                       <  1:480540/81‑1 (MQ=255)                               ggAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCa                                        >  1:480596/1‑79 (MQ=255)                                gAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCagag                                     <  1:480541/81‑1 (MQ=255)                                gAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCagag                                     >  1:480599/1‑81 (MQ=255)                                 aaaGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAgg                                    <  1:480542/81‑1 (MQ=255)                                     tttGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggcggc                                <  1:480543/81‑1 (MQ=255)                                         aTGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGaaa                            >  2:480591/1‑81 (MQ=255)                                               ttGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                       >  1:480617/1‑80 (MQ=255)                                               ttGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                       >  1:480590/1‑80 (MQ=255)                                                tGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                       >  2:480601/1‑79 (MQ=255)                                                  atatCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCagcagc                   >  1:480597/1‑81 (MQ=255)                                                  atatCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCagcagc                   >  1:480604/1‑81 (MQ=255)                                                  atatCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCagcagc                   >  1:480622/1‑81 (MQ=255)                                                    atCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAgcg                  >  1:480615/1‑80 (MQ=255)                                                     tCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAgcgc                 <  1:480544/80‑1 (MQ=255)                                                      cGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCaa               >  1:480608/1‑81 (MQ=255)                                                       gCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCaa               <  1:480545/80‑1 (MQ=255)                                                         cAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCg            <  1:480546/81‑1 (MQ=255)                                                            tttGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGgcg         >  2:480592/1‑81 (MQ=255)                                                             ttGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGgcg         >  2:480630/1‑80 (MQ=255)                                                              tgtgTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGgcgc        <  1:480547/80‑1 (MQ=255)                                                               gtgtTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCa       <  1:480548/80‑1 (MQ=255)                                                                tgtTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACg     >  1:480609/1‑81 (MQ=255)                                                                  ttGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCt   <  1:480550/81‑1 (MQ=255)                                                                  ttGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCt   <  1:480549/81‑1 (MQ=255)                                                                    gcgccgATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTc  <  2:480551/80‑1 (MQ=255)                                                                       |                                                                             AGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTC  >  NC_000913/2693303‑2693449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑